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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20151
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Subject: Agricultural Biotechnology × Author: Deka, Diganta × End date: 2017 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    STUDY OF DIFFERENTIAL GENE EXPRESSION IN RICE UNDER DROUGHT STRESS
    (AAU, Jorhat, 2015-07) Deka, Diganta; Modi, M. K.
    Rice is a dietary staple for a large part of the world’s human population which is grown under varying water regimes ranging from flooded to rainfed upland condition. The predominantly rice-growing areas in Asia are often threatened by severe abiotic stresses, the most common being drought which affects the yield potential of rice across all agro-climatic regions of the globe. Interestingly, some of the rice varieties of Northeast India are found to be drought tolerant e.g. Banglami. Comparative biochemical and physiological analyses of the variety with a high-yielding variety Luit confirmed this. Keeping all these points in view, high throughput RNA-Seq of the variety Banglami in presence and absence of drought was performed as an attempt to study the differential gene expression in Banglami. The RNA isolated by Trizol reagent (Invitrogen) was used for preparation of paired-end libraries using Illumina TruSeq RNA Library Preparation Kit. Libraries were sequenced using 2 X 150 PE chemistry on NextSeq. The reads were aligned against the indica reference assembly (ASM465v1). The expression analysis of the genes revealed 25,272 and 24,408 numbers of expressed genes in well watered control and drought stressed sample, respectively. Further analysis revealed 391 numbers of genes showing differential expression among which 86 were up-regulated and 305 were down-regulated. Among the differential expressed genes a number of genes were found to be very important for development of drought stress tolerant behaviour. Further, downstream analysis like Gene Ontology enrichment analysis, KEGG pathway analysis and QTL mapping were also performed which revealed important informations regarding the differentially expressed genes under drought stress condition in particular and the whole transcriptome of the Banglami variety of rice in general. The present study identified altered gene expression in rice induced by drought stress and provided a comprehensive map of drought responsive genes and pathways. Thus the results of the present investigation can serve as valuable genetic resource for gene expression, genomics and functional genomics studies in general and drought stress research in rice in particular.