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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20231
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Subject: Agricultural Biotechnology × Author: Das, Ratnakishore × End date: 2023 × Start date: 2023 × Type: Thesis ×
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    ThesisItemOpen Access
    STUDY ON GENETIC FIDELITY IN TISSUE CULTURED RAISED PLANTLETS OF CITRUS LIMON (ASSAM LEMON)
    (2023) Das, Ratnakishore; Sen, Priyabrata
    Citrus lemon, a highly significant species both economically and nutritionally, is extensively cultivated in Assam. The demand for this valuable crop has led to the development of efficient methods for large-scale multiplication to meet the increasing requirements of the market. Micropropagation, an advanced tissue culture technique, offers a promising approach for the rapid production of genetically identical plants. In this study, Citrus lemon was micropropagated to assess its potential for large-scale multiplication, and the genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR), Inter-Simple Sequence Repeat (ISSR), and long Retrotransposon (LTR) markers. SSR markers are highly polymorphic, consisting of short repetitive DNA sequences, while ISSR markers target the regions between microsatellites. LTR markers, on the other hand, focus on retrotransposon dispersed throughout the genome Healthy and vigorously growing shoot-tip were excised from donor plants and placed on the medium. Within a few weeks, these shoots successfully developed into multiple plantlets through the process of organogenesis. The micropropagated plantlets were then acclimatized to the greenhouse environment, and their growth performance was monitored. To determine the genetic stability and fidelity of the micropropagated plantlets. Fifty plant samples including the mother plant and rest in vitro regenerated plantlets are used for testing the genetic fidelity. Total genomic DNA was extracted from both the mother plants and the micropropagated plantlets using standard protocols, and the SSR, ISSR, and LTR markers were employed to assess genetic variation, if any, between the two sets of samples. The results of the genetic fidelity analysis demonstrated no detectable variation between the micropropagated Citrus lemon plantlets and their mother plants. All the SSR, ISSR, and LTR markers revealed identical banding patterns, indicating the absence of any somaclonal variation during the micropropagation process. The amplified fragments of the micropropagated plantlets precisely matched those of the donor plants, confirming the maintenance of genetic uniformity in the micropropagation protocol. In conclusion, this study demonstrates that, successful micropropagation of Citrus lemon with retained genetic fidelity is of immense significance for large-scale multiplication and commercial production.