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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    IDENTIFICATION AND VALIDATION OF DROUGHT-RESPONSIVE GENES IN THE UPLAND RICE CULTIVAR ‘BANGLAMI
    (AAU, Jorhat, 2020-10) Borah, Akankshita; Modi, M.K.
    Drought is one of the most important and highly unpredictable abiotic stresses causing drastic reductions in yield under rainfed rice environments, affecting 20% of the total rice-growing area in Asia (Pandey and Bhandari, 2008). Assam has diverse germplasm of rice which includes Banglami, a traditional drought-tolerant Ahu rice cultivar found locally in the state. Since 2013, crossing has been carried out between this drought-tolerant cultivar and Ranjit, a high yielding but drought-susceptible elite cultivar. Many drought-related QTLs were identified and reported from this cross-population. For the current study, twenty QTLs identified in the F4 population were selected for mapping on the parental genome. The results revealed that 17 of the 20 QTLs were present on the parent genomes where 11 of the 20 QTLs (qEBT3.1, qEBT6.2, qNOT6.1, qNOT2.1, qPL1.1, qPL1.2, qPL9.1, qPL9.2, qPL1.1, qNOG9.1, qNOG12.1) were found common to both the cultivars. From these, two of the QTLs (qGY1.1, qGY7.1) were found to be exclusively contributed by Banglami while four of the QTLs (qRLWC9.1, qRLWC9.2, qPH7.1, qDTF12.2) were found to be contributed by Ranjit to the cross-population. Genes associated with the QTLs were identified from whole-genome sequence data of both the cultivars. In total, 2454 genes were located in the 13 QTLs found in Banglami while 1585 genes were found in Ranjit in the 15 QTLs. The differential expression of the candidate genes in water-stress and irrigated conditions were calculated with the help of available RNA-seq data. Differential gene expression was calculated for each of the candidate genes under drought and control. Fifty DEGs in Banglami and seventy-two DEGs in Ranjit having a Log2 Fold change greater than ± 2.5 up to ± infinity were considered significant. Few of the differentially expressed genes having a significant Log2Fold change as revealed by the sequencing data, were confirmed experimentally under drought situation using qRT-PCR. Stress-responsive candidate genes involved in nitrate uptake, DNA repair, ubiquitination, enzymatic activities, cell cycle activities, etc. which can play a significant role in conferring drought tolerance were identified in the drought-related QTLs. The expression pattern of the candidate genes in qRT-PCR was found similar to transcriptome studies, signifying important role of the candidate genes in drought tolerance mechanism in rice.