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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20231
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Subject: Agricultural Biotechnology × Author: Bora, Smriti Shyamolee × End date: 2023 × Start date: 2023 × Type: Thesis ×
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    ThesisItemOpen Access
    GENE EXPRESSION STUDIES FOR LOW TEMPERATURE TOLERANCE AT THE BOOTING STAGE IN RICE
    (2023) Bora, Smriti Shyamolee; Baruah, Akhil Ranjan
    Rice (Oryza sativa L.) is a staple food crop for half of the world’s population and it has been grown as major cereal crop in three distinct seasons in Assam. Out of various constrains of rice production, abiotic stresses such as drought, low temperature (LT), salinity adversely affect crop growth and development. Low temperature stress at the early growth and reproductive stages in rice are critical, and the present study was conducted to screen low temperature tolerant genotypes at the booting stage (intermediate stage of reproductive phase) and study gene expression for cold responsive genes in tolerant and susceptible rice genotypes. A total of 30 rice cultivars collected from Regional Agricultural Research Station, Karimganj, AAU were subjected to low temperature stress at the booting stage in a growth chamber maintained at 12- 12.5C for seven days. The recovery and low temperature tolerance of plants after stress were evaluated based on pollen fertility, number of panicle emerged, number of seeds set and grain yield. The results revealed that the phenotypes of C-59 and IET-9097 were superior for all the traits under study whereas Costco and IET-8687 showed lowest trait values, indicating varied levels of low temperature tolerance at the booting stage. These four cultivars, C-59 and IET- 9097 as low temperature tolerant; Costco and IET-8687 as low temperature susceptible were chosen to study gene expression analysis using six cold responsive genes (OsCOLD1, OsDREB2A, OsCTB1, OsLTT1, OsAPX1, OsNAC9) through real time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). The results showed that all the genes were up-regulated in the cold tolerant cultivars, C-59 and IET-9097 and down regulation in the other two cold susceptible cultivars, Costco and IET-8687. After performing qRT-PCR analysis, correlation between the gene expression data and phenotypic observations followed by path analysis was performed to detect associations between the genes and phenotypes. Path analysis revealed that out of six genes used in the study, three genes showed direct positive effect over grain yield (OsDREB2A, OsCTB1 and OsNAC9) and three genes showed indirect effect for grain yield (OsCOLD1, OsLTT1 and OsAPX1). The study is preliminary with respect to low temperature tolerance at the booting stage using rice collection of AAU and hence, further validation is required.