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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

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End date: 2018 × Start date: 2017 × Type: Thesis × Rating Count: 4 ×
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    ThesisItemOpen Access
    DETECTION OF ANTIFUNGAL POTENCY OF WEED EXTRACT AGAINST PHYTOPATHOGENIC FUNGI.
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2018-01-24) SHITOLE, AMOL VIJAY; Gade, Dr. R. M.
    The present investigation on “Detection of antifungal potency of weed extract against phytopathogenic fungi” was conducted during 2013-2017, which included isolation and pathogenicity of fungal pathogens, screening of extracts of weeds by using different solvents viz., acetone, chloroform, sterilized double distilled water, ethanol and methanol. Bioagents and fungicides were also used against selected pathogens, compatibility of bioagents and weed extracts, seedling vigour index of tomato and chickpea cultivars, effect of weed extracts, bioagents alone and in combination on damping off of tomato and wilt and root rot incidence of chickpea under pot culture and phytochemical analysis, TLC, bioautography and partial structure elucidation of antifungal compounds by using FT-IR, GC-MS and NMR techniques from selected weed extracts. The pathogenic ability of Pythium debaryanum, Aspergillus niger, Sclerotium rolfsii and Fusarium oxysporum f. sp. ciceri was proved under pot culture by using sick soil technique. Among all the solvent extracts, methanolic extract of Psoralea corylifolia L. seeds (Bavanchi) had potential antifungal activity against Pythium debaryanum, Aspergillus niger, Sclerotium rolfsii and Fusarium oxysporum f. sp. ciceri. P. fluorescens and T. harzianum were found compatible with each other and also with methanol extract of Psoralea corylifolia seeds. Different solvents viz., acetone, chloroform, double distilled water; ethanol and methanol were tested to compare the maximum extraction yield from four different weeds. Methanol was found useful for getting highest per cent extraction yield. Psoralea corylifolia seeds methanol extract was selected for chromatographic analysis on the basis of in vitro growth inhibition assay against tested pathogens. This method was used to study the preliminary phytochemicals present in methanol extracts of four different weeds, while thin layer chromatography was used for separation of different compounds present in crude methanol extract and bioautography for isolation of active compound (s). Various analytical techniques viz., FT-IR, GC-MS and NMR were used for identification of active compounds in respective methanol extracts of Psoralea corylifolia seeds. All the weed extracts showed presence of alkaloids, cardio glycosides, fixed oils and fats, saponins, steroids and sterols, tannins and phenolics compounds. Further, fixed oils and fats were absent in D. stramonium and L. camara leaves methanol extracts. Among nineteen different combinations of solvent systems used under experiment, toluene: ethyl acetate: methanol (24:4:2) was effective for good separation of compounds detected by thin layer chromatography. In bioautography assay, methanol extracts of Psoralea corylifolia seeds gave two promising compounds which were active against P. debaryanum, A. niger and F. oxysporum f. sp. ciceri. These metabolites which showed promising results were selected for analyzing structural elucidation using spectroscopic techniques viz., GC-MS, FT-IR and NMR (1H and 13C) which were useful for detection of mass of the compound, functional groups and carbon and hydrogen position within the compound, respectively. It is revealed from analytical data and mass library search that partial or most probable structure of compound one isolated from Psoralea corylifolia seeds was Phenol, 4-(3,7-dimethyl-3-ethenylocta-1,6-dienyl) and 7H-Furo [3,2-g][1] Benzopyran 7-One as compound two isolated from Psoralea corylifolia seeds at Rf values 0.87 and 0.47, respectively. Seed treatment with P. fluorescens 10 g/kg seed + T. harzianum 4 g/kg seed + Psoralea corylifolia seeds methanol extract 4% was proved effective to increase seedling vigor index in both tomato and chickpea in paper towel assay and also found effective to reduce incidence of damping off caused by P. debaryanum, chickpea wilt and root rot disease caused by F. oxysporum f. sp. ciceri and S. rolfsii.