Thumbnail Image

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

Browse

20171
Reset filters
Subject: Agricultural Biotechnology × Author: MALKOTE, MANIK MADHAVRAO. × End date: 2018 × Start date: 2010 × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    IN VITRO SHOOT TIP GRAFTING IN CITRUS FOR QUALITY SEEDLING PRODUCTION.
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-10-10) MALKOTE, MANIK MADHAVRAO.; Akhare, Dr. A. A.
    The present investigation entitled “In Vitro Shoot tip grafting in citrus for quality seedling production” was conducted at Centre of Excellence In Plant Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist.Akola (M.S.) during the academic year 2015-2017 with the objectives to study the effect of different parameters influencing shoot tip grafting efficiency and efficacy. The experimental material of present investigation comprised the three varieties of citrus viz., Rangpur lime, Jambhiri as rootstocks and Sweet orange as scion. For micropropagation of Sweet orange field collected nodal explants and epycotyle segments from in vitro grown seedlings was used. The multiples obtained were then used for grafting purpose on Jambhiri and Rangpur lime. When seeds of Jambhiri, Rangpur lime and Sweet orange were inoculated for germination on various media combinations, The highest % of germination was found on MS media suplimented with 6% sucrose along with GA3 @ 1mg/l. The highest percent germination in case of Jambhiri and Sweet orange was 86.66% and in case of Rangpur the precent germination was 80.33%. For the preparation of scion, micropropagation of Sweet orange explants viz. nodal explants from field and epicotyls segment from in vitro germinated seedlings were tried on various concentrations of BAP @ 1, 2, 3 mg/l and NAA @ 0.5 and 1 mg/l alone or in combinations. In case of field collected nodal explants the highest success percentage was 66.66% in 6 days where as vitro derived explants respond best on media combination MS + BAP @ 3mg/l in 6.33 days. In order to optimize shoot tip grafting protocol various parameters such as sucrose concentration, age of rootstocks effect of PGRs, size of scion etc. were standardized. Amongst which sucrose % from 1.5 to 6% were tried, out of which 6% sucrose showed highest number of successful grafts on both the rootstocks Rangpur lime and Jambhiri. The rootstock age was tried from 5 to 25 days after germination and highest % success 71.42% and 62.50% in Jambhiri and Rangpur respectively was recorded when 15 days old rootstocks was used. Different PGRs viz. kinetin (1 mg/l and 5 mg/l) and BAP (1mg/l and 5mg/l) were tried in order to enhance graft union and scion growth, the highest % success of graft was noted when scion were treated with BAP @ 1mg/l prior to graft it on rootstock either Rangpur lime (66.66%) Jambhiri (73.33%). The scion size tried varies from 0.2 to 0.7 cm amongst which the highest % of success was shown by 0.6 to 0.7 mm scions. In case of Rangpur lime the % success of graft was 58.33% where as 8.033% success was recorded in Jambhiri rootstocks.