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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola

Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme. The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered. The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers. In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.

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20171
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Subject: Agricultural Biotechnology × Author: LUNGE, AKSHAY RAJENDRA × End date: 2018 × Start date: 2017 ×
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    ThesisItemOpen Access
    IN-PLANTA TRANSFORMATION OF SOYBEAN (Glycine max L.) TO ENHANCE SALINITY AND DROUGHT TOLERANCE
    (Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2017-10-10) LUNGE, AKSHAY RAJENDRA; Jadhav, Dr. P. V.
    In the present investigation entitled “In-Planta transformation of soybean (Glycine max L.) to enhance salinity and drought tolerance” was under taken with the view to develop In-planta regeneration system for elite soybean genotype. The study was carried out at Biotechnology Centre, Dr. PDKV, Akola during year 2015-16. In vitro culture and genetic transformation of soybean is difficult due to its recalcitrant nature. Establishment of gene transfer procedure is prerequisite to devolved transgenic plant of soybean in a shorter period. Therefore, the transformation was perform using the most popular soybean cv., JS-335 to optimize the factor influencing transformation efficiency through Agrobacterium tumefaciens based in-planta transformation using LBA4404 strain harboring gene, PDH45. In the present study four strategies were employed for in-planta transformation i.e. infection to seed, infection to seed and then incubation for 24 h in Agrobacterium, infection to every developing stage and infection to flowering and pod filling stage. Several parameters, such as optical density, incubation time, acetosyringone concentration influencing In-planta transformation, have been evaluated in this study. In seed radicle treatment, number of flower and pod formation was highest among the treatments. All the plant samples (T1) tested were found to be negative after PCR confirmation with gene specific primer. Similarly, in treatment injected to seed radicle and incubated for 30 min. out of 50 sample two samples were found to be positive after PCR analysis with gene specific primer. Putative transgenics were screened by PCR confirmation. It helped to eliminate non-transgenic. The method also decreased the time and effort involved in the screening process The procured plasmid containing PDH 45 gene was transformed into the Agrobacterium using In-planta method without using helper strain and confirmed by restriction digestion and the gene specific set of primers.