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Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur

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20151
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Subject: Molecultar Biology and Biotechnology × End date: 2016 × Start date: 2012 × Type: Thesis ×
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    ThesisItemOpen Access
    Studies on the effect of cytokinin on direct regeneration ability from different explants in vigna umbellata (Thunb.)
    (JNKVV, 2015) Prajapati, Umashanker; Tiwari, S.
    Abstract In process to develop a direct regeneration protocol for ricebean [Vigna umbellata (Thunb.)] Ohwi and Ohashi cv. PRR-2 studies on influence of different explants and various plant growth regulators were undertaken. To evaluate the effect of cytokinin on in vitro regeneration from different explants namely nodal segment with axillary buds, stem segment with apical meristems, preconditioned stem segment with apical meristem and embryonic axis were cultured. During present investigation, stem segment with axillary bud explants were found to be the most responsive for induction of multiple shoots. MS medium fortified with 5 mg/l BAP exhibited maximum regeneration response (95.56±1.3%) with 6.42±1.0 shoots per explant. Shoot clusters obtained in initial cultures were elongated on medium fortified with BAP and GA3. MS medium with 0.3 mg/l BAP resulted in more than 5 fold increase of shoot length (6.55±0.92cm). Elongated shoots were transferred to root induction medium fortified with different auxins in varying concentrations. Medium with 0.50 mg/l IBA exhibited maximum root induction (91.22±3.90) with 9.17±0.13 roots per shoot and 7.35±2.94cm average root length. After successful root induction, plantlets were carefully removed from the culture bottles and washed gently to remove adhered medium. Plantlets were treated with 0.5% Bavistin® solution and transferred in plastic pots containing a sterile mixture of vermicompost, sand and soil (1:1:1). Each plant was covered with a polythene bag to ensure high humidity. The 85% of plantlets survived and resumed normal growth after one month of transplantation. Present study reveals that an effectual protocol is now available for viable plant regeneration which can be very efficiently used for multiplication of genetically important materials and for genetic transformation of ricebean.