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Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur

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20131
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Subject: Molecular Biology and Biotechnology × Author: Kale, Sumit Prakashrao × End date: 2015 × Start date: 2013 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Molecular diversity analysis of whitefly (Bemisia tabaci) collected from different regions of madhya pradesh
    (JNKVV, 2013) Kale, Sumit Prakashrao; Tiwari, S.
    ABSRACT The present study entitles “Molecular diversity analysis of whitefly (Bemisia tabaci) collected from different regions of Madhya Pradesh” was carried out with the objectives to standardize genomic DNA isolation method and analyze the genetic diversity of whitefly using DNA fingerprinting. This study aimed to employ DNA based method to clarify the existing of biotype among whitefly populations in East Madhya Pradesh, India. The genomic DNA of whitefly collected from different geographical regions of East Madhya Pradesh was isolated by standardize protocol (SDS + Proteinase K) produce intact band with high molecular weight. The mtCOI primer was used for identification of B biotype. Amplified products were resolved by electrophoresis on 1.2% agarose gel and photographed under gel documentation system. Specific mtCOI primer CI-J-2195 and L2-N-3014 showed the presence of ~880bp bands which confirmed the presence of B biotype in different geographical regions of East Madhya Pradesh. All identified B biotypes were used for molecular diversity analysis. The DNA samples were amplified with 5 RAPD primers in thermal cycler. Amplified products were resolved by electrophoresis on 1.2% agarose gel and photographed under gel documentation system. Four decamer primers amplified 8 RAPD marker loci. All 8 bands scored by RAPD markers found to be monomorphic. Average number of bands per primer was 2.00. One RAPD primer is not amplified in all population. No variation was found among whitefly samples collected from different geographical regions of East Madhya Pradesh. Its seems that B. tabaci found in there region of collection are of conserved type.