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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    Molecular characterization of fertility restorers for wild abortive rice cytoplasm
    (Rajendra Agricultural University, Pusa (Samastipur), 2015) Kumar, Alok; Sharma, V. K.
    A study was conducted to evaluate the microsatellite markers based polymorphism for identification of polymorphic and informative markers in order to characterize two WA type CMS lines, six fertility restorer lines, twelve experimental hybrids and F2 individuals of cross combination IR80559A x PRR78 to assess the genetic effects of fertility restorer genes in relation to fertility restoration and to validate the association of molecular markers with fertility restoration of cytoplasmic male sterile lines. The experimental hybrids and F2 individuals were evaluated on the basis of pollen and spikelet fertility to assess the extent of fertility restoration. Using a set of 24 microsatellite primer pairs for molecular characterization of CMS lines, fertility restorers and experimental hybrids, amplified products were generated and polymorphism was recognized on the basis of presence or absence of bands, besides variation in number and position of bands. Altogether 142 allelic variants were detected at 37 loci with an average of 3.83 alleles per locus. A total of 46 shared and 96 unique allelic variants were generated. The number of unique alleles per primer pairs ranged from one out of three alleles in the case of RM 315 to eight out of nine alleles in the cases of RM 216 and RM 6100. Remarkably higher polymorphism per cent was exhibited by primer pairs RM 216, RM 6100, RM 17, RM 591, RM 5359, RM 206, RM 171, RM 3233, RM 443, RM 1108 and RM 10318. Considering the number of alleles generated in conjunction with the level of polymorphism detected, the primer pairs RM 216, RM 6100, RM 17, RM 206, RM 171, RM 1108, RM 3233 and RM 10318 appeared to be highly polymorphic and comparatively more informative for the purpose of molecular characterization of entries under evaluation. These primers generated considerably greater number of allelic and polymorphic variants due to variation in the length of simple sequence repeats.