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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    Assessment of spot blotch resistance in wheat genotypes through tissue culture and molecular marker
    (DRPCAU, Pusa, 2021) Deepti; Sharma, Vinay Kumar
    An investigation was conducted to determine the percentage of formation of callus and regeneration of callus in selected twelve genotypes of wheat, namely, PBW-343, Chiriya-3, Sonalika, HD- 2967, Agra- local, yangmai#6, K-307, UP-2565, HD-3086, HD-2733, Salembo and Cuo/79/Prulla by using mature embryo. The best media for callogenesis was WM11[MS + 2,4-D (4.0 mgl-1) + NAA (2 mgl-1)] with remarkably higher frequency obtained (90.78%) and the best media WM34 [MS + 2,4-D (0.1 mgl-1) + Zeatin (5.0 mgl-1) +CuSO4 (12 mg)] resulted in 93.43% caulogenesis, whereas 90.65% was noted in the medium WM36 [MS + IBA (0.5 mgl-1)] for rhizogenesis. The in-vitro screening was performed and assayed by using culture filtrate of Bipolaris sorokiniana as a fungal toxin in the media selected for callogenesis and regeneration to facilitate precise evaluation for presence or absence of spot blotch or infection in wheat genotypes and to derive inference about their susceptibleness or resistance. The susceptible genotype Agra local showed maximum necrosis of the callus because of effect of toxin in medium supplemented with MS+2,4-D (4.0 mgl-1) + NAA (2 mgl-1) along with B. sorokiniana toxin, whereas the resistant genotype Yangmai#6 showed least area affected by the toxin as observed in the case of controlled medium supplemented with toxin. The utilization of twenty-two SSR and ten STS microsatellite markers known to be associated with resistance to spot blotch during amplification profiling of thirty-six genotypes of wheat under evaluation in the present investigation resulted in highly effective categorization of these genotypes having resistant, moderately resistant and susceptible response. Hierarchical cluster analysis using Sequence Tagged Sites specific primers exhibited a very high efficiency (91.7%) in discriminating the susceptible genotypes from resistant and moderately resistant genotypes. However, principal coordinate analysis exhibited clearly recognizable spatial distance between the susceptible and resistant genotypes, which were basically classified into two groups. Hierarchical clustering and spatial distribution pattern based on the amplification profile generated by utilization of Simple Sequence Repeats specific markers univocally discriminated the susceptible genotypes from moderately resistant and resistant genotypes. The entries were broadly classified into three groups. A combination of SSR and STS markers unambiguously differentiated the susceptible genotypes from moderately tolerant and highly tolerant genotypes. Principal coordinate analysis based spatial distribution pattern of the genotypes as well as radial tree diagram and factorial analysis completely corroborated the results obtained from dendrogram and the genotypes were found to be clustered into three groups in all the cases. The inference derived from the results of genetic structure analysis revealed that the genetic compositions with respect to the targeted genomic regions of the genotypes are basically the admixtures of different combinations of three ancestral components. Experimental results provided the evidences to infer that ample differentiation and divergence were revealed amongst the genotypes by utilization of spot blotch resistance related molecular markers.