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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    In vitro screening and induction of resistance against brown spot disease of rice caused by Helminthosporium oryzae
    (Rajendra Agricultural University, Pusa (Samastipur), 2015) Agarwal, Ruchi; Kumar, Harsh
    Copious callus formation from cultured dehusked seeds and high frequency and number of regenerated plantlets from subcultured seed callus through caulogenesis followed by rhizogenesis or through somatic embryogenesis were achieved in six selected rice genotypes namely Pankaj, Rajendra Bhagwati, Rajshree, RAU-Aer03, RAU-Aer04 and Sugandha using eleven different media with MS basal salts supplemented with various concentrations and combinations of plant growth regulators NAA, 2,4-D, BAP, KIN and TDZ. The work resulted in efficient protocol for callus formation and plant regeneration. In vitro screening of selected genotypes for resistance/tolerance to brown spot disease through callus growth and shoot differentiation in presence of culture filtrate ranked the selected genotypes for their relative resistance to disease. The callus development and shoot differentiation in presence of high concentration of culture filtrate indicated the induction of resistance / tolerance to disease. Molecular marker studies were also done for assessment of genetic variability with respect to brown spot disease resistance. Utilizing a panel of ten SSR primers linked to brown spot disease namely, RM 21, RM 206, RM 229, RM 257, RM 263, RM 566, RM 1367, RM 3515, RM 3907 and RM 6499 in the study revealed remarkably higher level of genetic polymorphism which allowed unique genotyping of the entries and somaclonal variants. Clustering of genotypes based on amplification profiles differentiated resistant and moderately resistant entries from highly susceptible with respect to brown spot disease. The molecular marker study graded the six selected genotypes of rice for their resistance/tolerance to brown spot disease in the order: RAU-Aer04, Rajshree, Rajendra Bhagwati, RAU-Aer03, Sugandha and Pankaj which was the same as observed during in vitro screening.