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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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Now showing 1 - 9 of 14
  • ThesisItemOpen Access
    S election of native Trichoderma strain with Biocontrol potential from sugarcane rhizosystem
    (Dr.RPCAU, Pusa, 2021) Rajput, Virendra Singh; Dwivedi, Meenakshi
    In the currently studies, Trichoderma samples were isolated from rhizosystem of sugarcane crop. The isolates were vigorously tested for their Biocontrol potential. On the basis of their antagonistic potential against C. falcatum pathotypes Cf 07 and Cf 08, the promising isolates were selected. A total of 27 isolates each were obtained from samples from SRI, Pusa and Harpur Jhilli, Pusa. Based on visual cultural differences, 19 isolates were screened from SRI sample and 16 isolates were from Harpur Jhilli sample. Out of the isolates, T SR 11, T HJ 7, T HJ 14 and T. asperellum stronglyinhibited the growth of the pathogen in vitro. The isolates were then dual cultured with the pathotypes in the presence of fungicides (carbendazim 50 WP and azoxystrobin 23 SC), in order to create a micro-environment so that all the three components can interact with each other at the same time. It was found that carbendazim completely inhibited Trichoderma isolates at all the doses (RD, ½ RD, ¼ RD). Hence it cannot be used with Trichoderma under IDM programme. Whereas, azoxystrobin 23 SC was found to be compatible with Trichoderma isolates. Moreover, it was found to aggravate the growth of Trichoderma isolates and suppress the growth of the pathogen. Inhibition of pathogen by ½ RD and ¼ RD in integrated approach was comparable with inhibition at RD of the fungicide. Hence, it was concluded that azoxystrobin 23 SC could be used in reduced doses (1/2 RD and ¼ RD) with BiocontrolTrichoderma isolates and still would be able to manage the pathogen effectively. Hence, it gives a hope that effective IDM strategy against rd rot could be chalked out including azoxystrobin and compatible BiocontrolTrichoderma isolates.
  • ThesisItemOpen Access
    Evaluation of button mushroom Agaricus bisporus (Lange)Imbach germplasm for yield potential under Bihar condition
    (DRPCAU, PUSA, 2021) P, RAMESH; Nandni, Sudha
    The present work entitled “Evaluation of button mushroom Agaricus bisporus (Lange)Imbach germplasm for yield potential under Bihar condition” was carried out at Advance Centre of Mushroom Research, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur,Bihar as well as Mushroom Unit, T.C.A., Dholi. The button mushroom germplasms viz., IVTB-18-01, IVTB-18-03, IVTB-18-05, IVTB-18-09 and the local strain AB-18-03 were evaluated for different growth characteristics including growth on different media viz., Potato Dextrose Agar (PDA), Malt Extract Agar (MEA), Yeast Extract Potato Dextrose Agar (YEPDA), and Compost Extract Agar (CEA) at 7th DAI and 14th DAI under invitro conditions. The Compost Extract Agar media showed the best mycelial growth i.e.39.7 and 86.9 mm after 7th DAI and 14th DAI respectively. The maximum colony diameter was observed in the germplasm IVTB-18-09 after 7th DAI (44.5 mm) and 14th DAI (90.0 mm). The minimum number of days for spawn run in wheat grains were recordedwithIVTB-18-03 (15 days) while spawn runin compost was recorded to be minimum withIVTB-18-09 (15 days). The germplasm IVTB-18-09 was observed with minimum number of days (10 days) taken for casing to pinhead formation, minimum number of days (18 days) for casing to first flush harvest, maximum pileus diameter (44.5 mm) and the highest stipe diameter(52.1 mm), while the germplasm IVTB-18-05 had a maximum stipe length of (20.2 mm). The highest number of fruiting bodies (167 nos.), yield (2298 g)and biological efficiency (32.8%) were obtained from the germplasm IVTB-18-09. The minimum green mould, bacterial blotch, and inky cap incidence occurred in the local strain AB-18-03. Finally concluded that the germplasm IVTB-18-09 wasfound to be suitable for cultivation under the Bihar condition.
  • ThesisItemOpen Access
    Eco-friendly Management of Xanthomonas campestris pv. campestris causing black rot of cauliflower
    (DRPCAU, PUSA, 2021) PATIDAR, RAHUL; Ranjan, Rajesh Kumar
    Black rot disease is a serious problem in the development of cauliflower, which is one of India's most popular winter vegetables. The pathogen for black rot disease was identified as Xanthomonas campestris pv. campestris through morphological and biochemical characteristics. Colony characters development on NSA medium regards to morphological characters. Biochemical test such as gram staining, indole production, nitrate reduction, KOH string test, catalase test and utilization of different carbohydrates for acid production regard as biochemical characters. In vitro evaluation of 30 rhizobacteria against XCC in which 3 isolates found effective and developed inhibition zone of more than 10 mm. RAC 3 isolates developed maximum inhibition zone of 27.03 mm. Aqueous extract of Neem, Garlic, Bael, Datura and Tulsi were effective at 1:1 (w/v). Garlic extract shows maximum inhibition zone of 13 mm and 15.23 mm at 10% and 15% conc respectively, followed by Neem (11.60 mm and 14.40 mm) and Datura (11.20 mm and 13.83 mm). Beal extract shows inhibition zone of 11.83 mm only at 15% conc and Tulsi failed to produced inhibition zone at all three conc. Evaluation of antibacterial chemical indicates that 0.01% conc. of streptomycin sulphate + tetracycline gave maximum inhibition zone of 22.43 mm followed by streptomycin alone (20.44 mm) and chloramphenicol (18 mm) but at higher conc., chloramphenicol @ 0.02 % and 0.03% developed maximum inhibition zone of 25.89 mm and 27.33 mm respectively, followed by streptomycin sulphate + tetracycline (23.89 mm and 26.32 mm) and streptomycin alone (21.33 mm and 23.87mm).
  • ThesisItemOpen Access
    STATUS OF FUNGAL DISEASES OF IMPORTANT MEDICINAL PLANTS IN SAMASTIPUR, BIHAR
    (DRPCAU, PUSA, 2021) SINGH, NIDHI; Rai, Dinesh
    India is a treasure house of medicinal plants and is endowed with precious genetic diversity. They are not an exception and are also attacked by several pathogens, mainly the fungal pathogens that degrade the quality of medicinal active principle which may lead to health hazards instead of curing the disease. A survey was carried out on fungal diseases of economically important five medicinal plants viz. Ashwagandha (Withania somnifera), Sarpagandha (Rauvolfia serpentina), Tulsi (Ocimum sanctum), Mint (Mentha arvensis) and Mandukparni (Centella asiatica) in MAP germplasm blocks at Dr. RPCAU, Pusa and its neighbouring areas at different time intervals. During the survey, eleven fungal diseases namely leaf spot/ blight (Alternaria alternata), wilt (Fusarium solani) and downy mildew (Peronospora sp.) of Ashwagandha, leaf spot (Colletotrichum truncatum) and leaf blight (Alternaria sp.) of Sarpagandha, leaf spot (Colletotrichum gloeosporioides) and leaf blight (Alternaria sp.) of Tulsi, leaf spot (Curvularia lunata) and leaf blight (Alternaria alternata) of Mint and leaf spots (Cochliobolus sp. and Alternaria sp.) of Mandukparni were recorded. There was an increase in disease incidence during November to January. The overall variation recorded in disease incidence of the medicinal plants varied from trace (wilt and downy mildew of Ashwagandha) to 60 per cent (leaf spot of Mandukparni and Sarpagandha). The PDI also varied from trace to 61.11 per cent among the plants. All the isolated pathogens varied significantly in most of their cultural and morphological characteristics. In vitro evaluation of native Trichoderma asperellum showed effective inhibition against the pathogens. Maximum inhibition percent was recorded against Fusarium solani i.e. 56.36 per cent followed by Cochliobolus sp. i.e. 55.38 per cent. Among the tested botanicals and essential oils, Garlic bulb extract was found most effective against most of the pathogens at both 5 and 10 per cent concentrations in showing complete inhibition (100%) followed by Neem leaf extract which showed maximum inhibition of pathogens (Alternaria spp., Fusarium solani, Colletotrichum truncatum, Curvularia lunata, Cochliobolus sp.) at 5 per cent concentration and Bael extract (Alternaria alternata, Cochliobolus sp., Colletotrichum gloeosporioides, Alternaria spp., Colletotrichum truncatum) at 10 per cent concentration. This work may encourage other researchers to study these diseases further and their integrated management using biocontrol agents and plant extracts by properly testing their efficacy in the agrifields.
  • ThesisItemOpen Access
    BIO-RATIONAL MANAGEMENT OF ALTERNARIA LEAF SPOT OF CAULIFLOWER
    (DRPCAU, PUSA, 2021) SUNITHA, MUMMINENI; Jha, Pk.
    Cauliflower, which is widely grown throughout the world, is one of the most popular vegetable in Brassicaceae family after cabbage in term of area and production across the globe. Alternaria leaf spot or Alternaria leaf blight, which is the most destructive disease of cauliflower crop at vegetable curd stage and seed setting stage, has been attributed to cause 30-50% losses and in most severe case, the losses in yield may reach up to 98% in seed crop. In the present investigation, the pathogen Alternaria brassicae was found to produce typical symptoms of the disease as small, irregular brown spots with concentric rings, also surrounded by yellow chlorotic halo. Later on lesions were found to coalesce forming irregular, brown to dark brown patches with concentric rings. It was also found to affect petiole, stem and curd at later stages. Severely affected seedlings showed dark brown spots with damping off symptoms. In current investigation, four biocontrol agents i.e. the native isolates of Trichoderma viride and Trichoderma asperellum as well as commercial formulations of Trichoderma viride and Trichoderma harzianum were evaluated for their antagonistic effect on A. brassicae. Among bio-agents, the native isolates of T. viride and T. asperellum were found very effective against Alternaria brassicae with 68.61% and 65.19% growth inhibition, respectively. Aqueous and ethanol extracts of six botanicals (Neem, Datura, Tulsi, Bael, Brahmi, Mandookparni) were tested against A. brassicae. The aqueous extracts of Neem at 10% concentration showed maximum inhibition (54.07%) of target pathogen followed by Datura, Bael. The ethanol extracts of all the test botanicals were able to suppress the growth of the pathogen at all concentrations. At 10% of concentration, Datura and Bael exhibited 100% growth inhibition, at 20% of concentration Tulsi, Bael and Datura were found to exhibit 100% growth inhibition, at 30% and 40% of concentration Tulsi, Neem, Bael and Datura exhibited 100% growth inhibition; while at 50% of concentration, Tulsi, Neem, Bael, Datura and Brahmi exhibited 100% growth inhibition. Nine chemicals were evaluated against A. brassicae by poison food technique at four concentrations of 100ppm, 250ppm, 500ppm and 1000ppm. At 100 ppm concentration Propiconazole showed maximum inhibition (76.66%) followed by Mancozeb. At 250ppm, 500ppm and 1000ppm, Propiconazole showed maximum growth inhibition (100%) of the pathogen. Different level of inoculum of Alternaria brassicae were evaluated to identify the quantity of inoculum in order to develop sick pot for successful disease development. The application of 5.0 gram of inoculum showed quite poor disease development. By increasing the quantity of inoculum, the disease severity (PDI) was found to increase. The inoculum level of 40g induced maximum disease severity (50.81% PDI), at early stages, some plants showed damping off symptoms and some were stunted. Initially symptoms were expressed as small, irregular brown spots, later on spots were merged to form irregular, dark brown patches with concentric rings. During investigation on integrated management under sick pot condition, the combination of T. viride (applied as soil treatment @ 10g/pot) along with spray of Neem (10%) after appearance initial symptom and spray of Azoxystrobin (0.1 %) after 7-day of Neem spray was found most effective in controlling the disease.
  • ThesisItemOpen Access
    Evaluation of Shiitake mushroom, Lentinula edodes (Berk.) Pegler” under Bihar condition
    (DRPCAU, Pusa, 2020) Verma, Inkresh Kumar; Dayaram
    The present study entitled “Evaluation of Shiitake mushroom, L. edodes (Berk.) Pegler” under Bihar condition was carried out in Advance Centre of Mushroon Research, Department of Plant Pathology, DRPCAU, Pusa, Samastipur, Bihar, under in vitro condition various culture media like PDA, SDEA, WSEA, MEA, WSEA+PSEA and LLEA were evaluated for their effect on growth of L. edodes. The PDA was evaluated for growth of L. edodes at various temp. regimes wiz. 150C, 200C, 250C, 300C. The result clearly revealed that mycelium growth varied significantly among the media and strains tested. The max. colony diameter of 86.67 mm for LE- 1501, 90.00 mm for LE-1502, 86.33 mm for LE-1503, 84.33 mm for LE-1504 and 84.00 mm for LE-1505 were recorded on PDA at 14 DAI. However, the PSEA and LLEA media proved to be least effective media for mycelia growth of shiitake. The temp. analysis of data revealed that 250C was the most preferred temp. for the optimum growth of all strains of L. edodes at 7 and 14 DAI. Three grains substrates wiz. Wheat, maize and paddy were evaluated for the spawn production of shiitake. The wheat grains were most suitable substrate whereas maize grains were least effective for spawn development of L. edodes. The six substrates such as maize cob, wheat straw, LL, WSE+PSE, SD and PS were tested for the growth and yield of L. edodes strains. The result revealed that min. spawn run period of 25.00 days, 23.00 days, 25.00 days and 28.33 days were observed with strain LE-1501, LE-1502, LE1503 and LE-1504 in SD substrate. However, the min. days for spwan run (28.67) were exhibited by strain LE-1505 in WS substrate. The min. bump formation period of 54.00 days, 52.67 days, 54.67days and 56.67 days were observed with the strains LE-1501, LE-1502, LE-1504 and LE-1505 respectively in the MC substrate. The min. basidiocarp formation period of 68.00 days, 65.00 days, 66.00 days, 68.00 days and 70.00 days were observed with the strain LE-1501, LE-1502, LE-1503, LE-1504 and LE-1505 respectively in the maize cob substrate. The max. number of sporocarp of 12.33, 9.33, 10.33 and 11.00 were recorded with strain LE-1502, LE-1503, LE-1504 and LE-1505 in SD substrate while LE-1501 showed its maximum number of sporocarp (9.00) in maize cob substrate. The max. total yield (483.33g /1000g) and biological efficiency (48.33%) of L. edodes strain (LE-1502) were obtained in maize cob substrate. However the lowest total yield (253.33g / 1000g) and biological efficiency (25.33%) were recorded in WS+PS substrate with starin LE-1502. The various pathogens like Penicillium spp., Trichoderma spp., Aspergillus niger and coprinus were observed during cultivation of L. edodes.
  • ThesisItemOpen Access
    Studies on variability and management of Alternaria spp. causing Leaf blight of oilseed brassica
    (DRPCAU, Pusa, 2020) T.N., Punith Gowda; Choudhary, C. S.
    In the world, Oilseed brassica, called as Rapeseed-Mustard is the third most important oilseed crop after soyabean and palm. Bihar produces 0.11 mt from an acreage of 0.09 mha with a productivity of 1241kg/ha. As far as 35- 45 % losses had been reported in Indian mustard, even more losses in rapeseed up to 70%, due to Alternaria leaf blight disease caused by Alternaria brassicae and Alternaria brassicicola. Survey had conducted for the natural occurrence of the disease prevailed in different districts of Bihar and in a range of 25.67 to 69.67 per cent disease incidence was recorded. The black points were the first seen symptoms on the lower leaves which coalesce to form spots of brown to black coloured lesions. Disease spreads rapidly forming circular spots on middle and upper leaves. Upon severity target board like concentric rings were formed which leads to defoliation of the leaves. Symptoms were seen on the stem and siliquae deteriorating the crop and causing damage to seeds. Disease samples were collected from the surveyed fields and isolation of the pathogen from the isolates had done on the PDA medium. Out of ten isolates collected, seven were identified as Alternaria brassicae and three were identified as A. brassicicola. Pure cultures were maintained for further studies of morphological and cultural variability among the isolates. Mycelial growth of the pathogen and colony characters were considered to study the morphological variations. Radial growth of mycelium varied from 53.0 to 77.2 mm in diameter and five different colony colors were observed among the pure cultures. Pure cultures of ten isolates were inoculated on the five different solid media to check the cultural variability. Radial growth of the mycelium varied on different media from 39.3 to 76.3 mm in diameter. Conidial dimensions were also measured to check the variability. The average conidial length, breadth and beak length varied from 36.5 to 183.0 μm, 6.55 to 22.0 μm and 2.8 to 63.35 μm respectively. Hence it was concluded from the experiment that morphological and cultural variability exists in the pathogen causing Alternaria blight in oilseed brassica. Some new fungicides had evaluated both in vitro and in vivo experiments for next line as management practices because some popular and effective fungicides may likely to banned in future. Among the six different fungicides tested in in vitro, Trifloxystrobin 50WG was found more efficient in controlling the pathogen. The experiment conducted with three different concentrations and found that the radial growth of mycelium inhibited by Trifloxystrobin 50WG was minimum at all concentration tested viz. 39.3 mm @ 250ppm, 24.7 mm @ 500 ppm and 0.7 mm @1000ppm. The field experiment had conducted with three replications under eight treatments with six fungicides. Plots treated with seed treatment of Carbendazim 50WP@2g/kg of seeds followed by foliar sprayed with Trifloxystrobin 50WG@1ml/l of water were found superior to the other treatments. Least PDI (20%.5) maximum 1000 seed weight (4.1g), highest yield (1430 kg/ha), highest yield increase over control (591.7kg/ha) and highest CB ratio (1:71) was recorded from this treatment.
  • ThesisItemOpen Access
    Identification and management of pathogen associated with wheat seeds
    (DRPCAU, Pusa, 2020) Yamleshwar; Ranjan, R. K.
    Wheat (Triticum aestivum L.) belonging to the family Graminae is a major cereal food crop in the world. Globally, wheat cultivated over an area of about 221.6 million hectare with an annual production of 750.4 million tons in 2016-17 (Anonymous 2018). India the second largest wheat producing country in the world after China has the production of about 98.61 million tons with an average productivity of 33.18 q/ha and cultivated over an area of about 29.78 million hectare (ICAR-Indian Institute of Wheat & Barley Research, 2018). The main wheat growing states in India are Uttar Pradesh, Punjab, Madhya Pradesh, Haryana, Rajasthan and Bihar. Bihar rank 6th in wheat production and cultivated over an area of about 2.04 million ha, production 5.74 million tonnes and productivity 28.16 q/ha (ICAR-Indian Institute of Wheat & Barley Research, 2018). Wheat crop is affected by approximately 120 different diseases, among them, 42 diseases are seed borne and 35 diseases are caused by fungi (Hasan et al., 2005). In India, the important seed borne diseases of wheat are rust, smut, bunt, leaf blight & ear cockle disease. Hence, the investigation on “Identification and management of pathogen associated with wheat seeds” has been undertaken to determine the quality and health status of wheat seeds produced by the farmers in Bihar and their management by chemical and botanical oils. I Total 70 seed samples of different variety were collected from the farmers of different villages of Samastipur and Muzaffarpur districts of Bihar state for studies of mycoflora associated with wheat seeds and their management. Collected seed samples were categorized in four categories i.e., Shrivelled, discoloured or black point, damaged and healthy seeds. In Samastipur seed samples, shrivelled, discoloured, damaged and healthy seeds were found 8.53%, 8.16%, 6.57% and 76.72% respectively. While in case of Muzaffarpur seed samples shrivelled, discoloured, damaged and healthy seeds were found 7.68%, 5.54%, 4.88% and 81.90% respectively. Study on germination percentage by standard paper towel method, in Samastipur and Muzaffarpur seed samples average seed germination percentage recorded were 72.87% and 74.47% respectively and seedling vigour were recorded 1923.56 and 2309.60 in Samastipur and Muzaffarpur seed samples respectively. Study on bunt and loose smut disease detection, all seed samples were free from bunt and loose smut disease. Three methods for isolation of seed associated mycoflora i.e., standard blotter paper method, standard agar plate method and standard paper towel method, were used. Five seed associated mycoflora i.e., Aspergillus sp., Alternaria triticina, Bipolaris sorokiniana, Fusarium moniliforme and Penicillium sp. were detected and isolated from Samastipur and Muzaffarpur seed samples. Aspergillus sp. and Alternaria triticina were found dominating fungus in all three methods. In study of different chemicals against isolated pathogens, Propiconazole found most effective against B. sorokininana, followed by Mancozeb and Mancozeb + Carbendazim. In case of Fusarium moniliforme Alternaria triticina and Penicillium sp., Propiconazole has shown highest percent inhibition of growth at lowest concentration. However, in case of Aspergillus sp, Mancozeb + Carbendazim found most effective followed by, Copper oxy chloride. Study on efficacy of different botanicals oils against isolated pathogens, lemongrass oil found most effective against B. sorokiniana. Neem oil found most effective to inhibit the growth of Fusarium moniliforme. Eucalyptus oil found most effective to inhibit the growth of Alternaria triticina at all concentrations. Neem oil found most effective to inhibit the growth of Apergillus sp. and Penicillium sp. at all concentration 100, 200 & 300ppm.
  • ThesisItemOpen Access
    Studies on efficacy of biocontrol agent and chemical fungicide against collar rot disease of elephant foot yam [Amorphophallus paeoniifolius (Dennst)]
    (DRPCAU, Pusa, 2020) Sankranti, Akshay; Mishra, A. K.
    Elephant foot yam [Amorphophallus paeoniifolius (Dennst)] is commonly known as ‘king of tuber crops’ due to its higher biological efficiency, cultivated commercially in the districts of Muzaffarpur, Samastipur, Vaishali, Bagusarai and East Champaran of Agro-climatic Zone-1 of Bihar and regarded as money spinning crop. Among various diseases affecting elephant foot yam, collar rot disease caused by Sclerotium rolfsii causes significant yield loss to the tune of 20 to cent per cent. Considering seriousness of this disease, present investigation was carried out in experimental plot Tirhut College of Agriculture, Dholi, Muzaffarpur (Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar) during 2019-2020. Three broad objectives i.e., survey for prevalence of collar rot disease in elephant foot yam, germplasm screening against collar rot disease and management of collar rot disease through biocontrol agent and chemical fungicide were considered during study. Survey was conducted twice in crop growth period in Samastipur and Muzaffarpur district. Elephant foot yam grown in Samastipur district was found more prone to collar rot disease compared to Muzaffarpur district with increase in incidence of disease during August to September. Twenty number of germplasm were screened against disease including elephant foot yam variety, Gajendra (check) against collar rot disease. Among the screened germplasm, two germplasm viz., TCA EFY-16 and TCA EFY-13 emerged as a resistant germplasm with no disease incidence (0%) and high value of growth attributing characters and yield of 46.04 and 44.97 t/ha respectively. Field efficacy of biocontrol agent and chemical fungicide against collar rot disease was evaluated wherein corm treated with Trichoderma @5g/kg corm + soil application with Trichoderma grown in FYM @ 2.5 kg/pit + soil application twice with Trichoderma grown in FYM @250g/ plant (T6) resulted in minimum disease incidence at different fortnight interval i.e, 5.55% (75 DAP), 7.84% (90 DAP), 6.38% (105 DAP) and 4.52% (120 DAP) with overall 22.22% disease incidence with maximum disease reduction over control (52.94%) and highest yield (34.85 t/ha). Feasibility of treatment in economic terms was calculated by incremental cost benefit ratio (ICBR). The highest ICBR of 1: 2.57 with net profit over control was realized as Rs 134294/ha in treatment where corm were treated with Trichoderma @5g/kg corm + soil application twice with Trichoderma grown in FYM @250g/ plant (T2).