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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    Characterization and management of Xanthomonas oryzae pv. oryzae causing Bacterial leaf blight of rice
    (DRPCAU, Pusa, 2020) Naveena, Sirivella; Rai, Bimla
    In the present research work, the pathogen, Xanthomonas oryzae pv.oryzae was isolated from the Bacterial leaf blight infected rice plants. Infected samples are collected from the Pusa field of Bihar. The pathogen was identified as Xanthomonas oryzae pv.oryzae through isolation study (colony characteristics on TZC media), ooze test, pathogenicity test and different biochemical test. The pathogen showed yellow, smooth, raised, buttery colonies on nutrient agar medium. The results of biochemical studies revealed that the isolated Xanthomonas oryzae pv.oryzae were gram-negative, rod shaped and the pathogen showed positive reactions for potassium hydroxide test, catalase test, production of hydrogen sulphide whereas it showed negative reaction for oxidase test, nitrate reduction test and indole test. A total of 14 rhizobacteria were isolated from rhizosphere of rice plant and were screened in vitro for their bio control against Xanthomonas oryzae pv.oryzae in which 4 isolates were effective.RRB-13(Pseudomonas) showed the highest inhibition zone (18.1mm) after 48hrs of incubation. Seven antibacterial compounds (3 antibiotics & 3 chemicals) were evaluated against Xanthomonas oryzae pv.oryzae. Among the antibiotics Streptomycin showed highest antibacterial activity at all the three concentrations (250ppm, 500ppm and 1000ppm) produced inhibition zone 18.43mm, 21.43mm and 25.43mm respectively. Among the three chemicals ,Tricyclazole showed highest inhibition zone of 10.7mm, 14.7mm and 17.7mm at 0.05%, 0.1% and 0.2% concentration. Nine aqueous and ethanol extracts of botanicals were studied in vitro against Xanthomonas oryzae pv.oryzae. Among aqueous extract, Neem (Azadirachta indica) at 100% concentration found to be very much effective and showed an inhibition zone of about 23.4 mm. Among all the nine botanicals tested, Neem showed the high inhibition zone at all the three concentrations (20%, 30% and 40%) of 16.7mm, 17.4mm and 18.4mm respectively. Among the 9 ethanol extract of botanicals tested at 100% concentration, Garlic (Allium sativum) showed the high inhibition zone of 25.1mm. The ethanol extract of botanicals tested, garlic showed the high inhibition zone at the three different concentrations (20%, 30% and 40%) of 17.4mm, 18.4mm and 19.4mm respectively. Six essential oils were tested in vitro for their antagonistic nature in contrary to Xanthomonas oryzae pv.oryzae. Among them, Lemon grass oil showed highest inhibitory activity at 2000ppm (16.76mm). Citronella oil gave the good result at 500ppm (12.1mm) and 1000ppm (13.4mm).