Thumbnail Image

Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

Browse

20191
Reset filters
Subject: Plant Breeding × Author: Kumari, Pooja × Start date: 2013 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Diversity in mid late Sugarcane clones and their molecular characterisation
    (Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar), 2019) Kumari, Pooja; Kumar, Balwant
    The present investigation entitle ‘Diversity in mid late sugarcane clones and their molecular characterisation’ was undertaken with thirteen mid-late maturing sugarcane which were planted during 2018 at Research Farm of RPCAU, Pusa, Bihar in randomized block design with three replications to assess the variability parameters viz, variance and coefficient of variances at phenotypic and genotypic level, heritability and genetic advance as per cent of mean, correlation between pair of productive characters its direct and indirect effects on sugar yield, extent of diversity among the clones and its molecular characterizations. Observations were recorded for cane and sugar yield attributing traits viz, germination percentage at 45 Days, number of tillers (000/ha) at 120 DAP, number of shoots (000/ha) at 240 days, plant height (cm) at harvest, cane diameter at harvest (cm), brix % at 10 month stage, pol % at 10 months stage, purity % at 10 months stage, CCS % at 10 month stage, brix % at 12 month stage, pol % at 12 months stage, purity % at 12 months stage, CCS % at 12 month stage, single cane weight at harvest, fiber % at harvest, extraction % at harvest, pol % in cane at harvest, cane yield (t/ha) and CCS t/ha (sugar yield) at harvest.Observed data were subjected to statistical analysis to fulfill the objectives under study. Analysis of variance showed highly significant difference for all the character indicating wide range of variability existing among the clones. The clones CoP2061 and CoLk15469 were performed best for sugar yield and its attributing traits. Higher numerical values of phenotypic variances were observed for all the traits with respect to its genotypic counterpart, similarly highermagnitude of PCV was recorded for all the traits with respect to GCV indicating greater environmental influence on these traits for total variation. High heritability coupled with high genetic advance as percent of means was observed for sugar yield, cane yield, germination % at 45 days, cane height, single cane weight at harvest and fiber % at harvest. Therefore, it indicates the presence of additive gene action forsimple Title of Thesis : “DIVERSITY IN MID LATE SUGARCANE CLONES AND THEIR MOLECULAR CHARACTERISATION” Name of Student : POOJA KUMARI Registration Number : M/PBG/129/2017-18 Year : 2019 Major Subject : Plant Breeding & Genetics Minor Subject : Agricultural Biotechnology and Molecular Biology Major Advisor : Dr. Balwant Kumar Sugarcane Research Institute, Pusa, Bihar. Total pages in thesis : 103 + xi (Bibliography) + 1-12(Appendix) selection based on phenotype for these characters might be effective method for sugarcane variety improvement breeding programme. At phenotypic and genotypic level the characters viz. germination % at 45 days, number of shoots at 240 days, no. of milliable canes at harvest, cane height at harvest, pol % in juice at 12 month, purity % at 12 month, CCS %12 month, brix at 12 month, single cane weight, extraction %, pol % in cane showed highly significant and positive correlation with CCS t/ha (sugar yield). At genotypic level, pol % in cane showed high and positive direct effect on sugar yield followed by brix % at 12 month stage, fiber % at harvest, CCS % at 12 month and brix% at 10 month while Pol % in juice at 12 month exhibited maximum negative direct effect on sugar yield followed by pol % in juice at 10 month stage , CCS % at 10 month stage.Maximum positive indirect effect effect exhibited by pol % at 12 month stage via.pol % in cane followed by via., CCS % at 12 month stage, fiber % at harvest and brix % at harvest. Maximum negative indirect effect exhibited by CCS % at 12 month stage followed by via. pol % in juice at 10 month. All the thirteen clones were grouped into three clusters among them only cluster I contain eleven clones while others two were monogenotypic. The maximum inter cluster distance was found between cluster I &II followed by cluster I & III and cluster II & III while intra cluster distance was exhibited only in cluster I. Fiber % at harvest had highest percentage contribution toward genetic divergence followed sugar yield, brix % at 12 month, single cane weight, pol % in cane at harvest, brix % at 10 month, extraction % at harvest, no. of tiller at 120 days, cane height, cane diameter, pol % at 10 month. Clustering pattern showed that the genetic diversity was more important than geographical diversity because genotypes belonging to different places of origin were also present in the same clusters.This indicated that selection of genotypes for these traits may be rewarding for future utilization in breeding programme. A total of 56 alleles were obtained out of which 41 shared and 15 unique allelic variants were generated in the form of amplified product by polymerase chain reaction using eleven primer pairs.The PIC values revealing allelic diversity and frequency among the genotypes varied from 0.36 in case of NKS 57 to 0.90 in case of NKS 1 with an average of 0.62. The similarity coefficients revealing genetic similarity with respect to size of the amplified products generated from targeted regions of the genome varied from 0.518 to 0.857 for the pair wise combinations amongst thirteen entries under evaluation. Two Clusters were obtained when phenon line was drawn at twenty five similarity units. Cluster I consist of ten genotypes. The tri-genotypic cluster II consist of three genotypes. When phenon level draw at fifty similarity unit cluster I and II further divided in sub -clusters.It is concluded that use of SSR marker is very reliable approach for identification of diverse genotype(s) where phenotypic similarity of the cultivars leads to difficulty while selections of parents for hybridization.