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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    Screening of Rapeseed-mustard Genotypes and Eco-friendly Management of Mustard Aphid, Lipaphis erysimi (Kalt.).
    (Dr.RPCAU, Pusa, 2022) KARTHIK, SOMALA; Mukherjee, U.
    The current investigations were conducted on “Screening of Rapeseed-mustard genotypes and eco-friendly management against mustard aphid, Lipaphis erysimi (Kalt.)” during 2019-2020 & 2020-21. The surveys conducted during the year 2020 across India, revealed that Lipaphis erysimi pseudobrassicae was present in all the 16 locations surveyed belonging to 13 states and one Union Territory at over altitude ranging from 40.42 m (Dariapur) to 980.80 m (Barapani). The selective RAPD primers viz., OPA-02, OPA-04, F2 and SSR primers viz., ApH04M, Ap-01 and ApH08M were considered as informative for genetic diversity analysis based on polymorphic band percentage (>70), higher PIC, EMR, MI and RP. DNA barcoding of 16 locations with universal primer (LCO1490- Forward and RHCO2- Reverse) employed were amplified at 657 bp of mtCOI gene in Lipaphis erysimi pseudobrassicae. The phylogenetic tree showed the clustering of all 16 locations with L. e. pseudobrassicae reference sequence. Among 16 locations, Jorhat of North-Eastern India was found to be highly diverged with higher branch distance (0.13) in the phylogenetic tree and higher dissimilarity of 30.0 and 31.0 % in RAPD and SSR dendrograms respectively. The association of bacterial endosymbionts with L. e. pseudobrassicae showed that Buchnera sp, a primary endosymbiont was found in all the 16 locations. Whereas in secondary endosymbionts, Wolbachia sp, Rickettsia sp, and Hamiltonella sp dominated the most with 81.25 % and Cardinium sp liberated with 31.25 %. Among all 16 locations, Jodhpur and Ranchi had a 100 % incidence of endosymbionts and lower incidence was found in Ludhiana, Raipur, Dharwad, Alipur Bihta, Delhi, Jorhat (42.85 %). The inspections on population of alate mustard aphids and natural enemies i.e., adult coccinellids, syrphids and parasitoids in relation to weather parameters revealed their peak on 8th SMW (157.1, 6.48, 5.63, 14.62/ 4 traps) respectively and shown strong positive correlation with maximum temperature (0.445, 0.608*, 0.595*, 0.618*) and sunshine hrs (0.602*, 0.654*, 0.633*. 0.662*) respectively. Different Rapeseed-mustard genotypes were screened against mustard aphid resulting in identification of six promising genotypes among which, genotype RTM 314 had shown low AII (1.3), ADI (1.0) and ARI (1.15). The biochemical analysis also revealed the highest glucosinolates (109.87 µmole/g) and phenols (6.51 mg/g) content in RTM 314. Further, GC-MS analysis indicated the existence of biologically active compounds like 2-(hydroxymethyl)-2-nitro-1,3-Propanediol; 5-(Methylsulfinyl)pentanenitrile and 1-Isothiocyanato-4-(methylsulfinyl)butane only in RTM 314 which could be the reason for resistance against mustard aphid. Among different intercrop combinations, mustard + wheat (3:1) followed by mustard + barley (3:1) have recorded lowest aphid population (41.46, 42.79/ top 10 cm inflorescence of 10 plants) respectively and highest population of coccinellids (8.29, 7.76/ 10 plants), syrphids (6.57, 6.35/ 10 plants), parasitoids (14.01, 13.34/ 10 plants) and spiders (4.39, 4.21/ 10 plants) with superior yield (1213, 1201 kg/ha) respectively. The efficacy of various botanicals was also evaluated against mustard aphid and its natural enemies. Among the botanicals tested on mustard aphid, the % reduction of mustard aphid population over untreated control following the first and the second spray was the highest in Neem Seed Kernel Extract (NSKE) @ 5% (70.69, 87.97 %) followed by Yam Bean Seed Extract (YBSE) @ 5% (66.58, 86.06 %) and Bhang Leaf Extract (BLE) @ 5% (64.25, 84.56 %) respectively besides with higher population of natural enemies. However, based on B: C ratio, BLE @ 5% with 6.86:1 was found to be better followed by NSKE @ 5% (3.61:1) and YBSE @ 5% (3.31:1).