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Dr. Rajendra Prasad Central Agricultural University, Pusa

In the imperial Gazetteer of India 1878, Pusa was recorded as a government estate of about 1350 acres in Darbhanba. It was acquired by East India Company for running a stud farm to supply better breed of horses mainly for the army. Frequent incidence of glanders disease (swelling of glands), mostly affecting the valuable imported bloodstock made the civil veterinary department to shift the entire stock out of Pusa. A British tobacco concern Beg Sutherland & co. got the estate on lease but it also left in 1897 abandoning the government estate of Pusa. Lord Mayo, The Viceroy and Governor General, had been repeatedly trying to get through his proposal for setting up a directorate general of Agriculture that would take care of the soil and its productivity, formulate newer techniques of cultivation, improve the quality of seeds and livestock and also arrange for imparting agricultural education. The government of India had invited a British expert. Dr. J. A. Voelcker who had submitted as report on the development of Indian agriculture. As a follow-up action, three experts in different fields were appointed for the first time during 1885 to 1895 namely, agricultural chemist (Dr. J. W. Leafer), cryptogamic botanist (Dr. R. A. Butler) and entomologist (Dr. H. Maxwell Lefroy) with headquarters at Dehradun (U.P.) in the forest Research Institute complex. Surprisingly, until now Pusa, which was destined to become the centre of agricultural revolution in the country, was lying as before an abandoned government estate. In 1898. Lord Curzon took over as the viceroy. A widely traveled person and an administrator, he salvaged out the earlier proposal and got London’s approval for the appointment of the inspector General of Agriculture to which the first incumbent Mr. J. Mollison (Dy. Director of Agriculture, Bombay) joined in 1901 with headquarters at Nagpur The then government of Bengal had mooted in 1902 a proposal to the centre for setting up a model cattle farm for improving the dilapidated condition of the livestock at Pusa estate where plenty of land, water and feed would be available, and with Mr. Mollison’s support this was accepted in principle. Around Pusa, there were many British planters and also an indigo research centre Dalsing Sarai (near Pusa). Mr. Mollison’s visits to this mini British kingdom and his strong recommendations. In favour of Pusa as the most ideal place for the Bengal government project obviously caught the attention for the viceroy.

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  • ThesisItemOpen Access
    DETECTION AND VALIDATION OF EST-SSR MARKERS ASSOCIATED WITH SUGAR YIELD IN SUGARCANE
    (DRPCAU, PUSA, 2022) S, DIVAKAR; SINGH, ASHUTOSH
    Sugarcane (Saccharum spp.) is a widely cultivated crop and fulfils around 75 % of sucrose demands worldwide. Due to its polyploidy and complex genetic nature, it is difficult to map the gene for sucrose content. But association mapping is one of the alternatives to identify the genes or markers for marker-assisted selection. In the present study total of 212 EST-SSR primers were collected from different literature. The functionality of each primer was tested by using Blast2Go software and 30 EST-SSR primers were selected that related with sugar content. These markers were validated by association mapping. Total 70 F1 diverse genotypes for sugar contents were phenotype with two check lines. All the parameters for sugar contents were recorded. The results showed a significant variation between the genotypes for sugar yield traits such as Brix value, purity, and sucrose content, etc. The correlation studies revealed that the Brix%, Sucrose content, and sucrose recovery were significantly correlated. The association analysis was performed by using MLM (Mixed Linear Model) and avoid false positive associations. The association analysis revealed that SEM 407 marker was significantly associated with Brix% and sucrose content. SEM 407 primers putative related with diphosphate-fructose-6-phosphate 1- phosphotransferase that are associated with Brix% and sucrose content. This functional marker can be used for marker assisted selection for sugar yield traits in sugarcane.