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Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Dist. - Nadia, West Bengal PIN - 741 252

The university established in 1974, has completed five decades of its existence as the pioneer institute of Agricultural Education, Research and Extension. The main objective of this Viswavidyalaya is to provide facilities for the study of Agriculture, Horticulture and Agricultural Engineering. It is also to conduct researches in these sciences and undertake the educational and extension programmes in agriculture among the rural clientele base, keeping in view the requirements of the state.

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20231
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Subject: Agricultural Biotechnology × Author: Saha Pratik × Start date: 1968 ×
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    ThesisItemOpen Access
    Development of genetic and genomic resources for Downy mildew resistance and low ODAP content in Lathyrus sativus L.
    (Agricultural Biotechnology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia – 741252, 2023-12-20) Saha Pratik; Dr. Md. Nasim Ali
    Grasspea (Lathyrus sativus L.) belongs to the family Fabaceae and the tribe Vicieae having chromosome number (2n = 2x = 14). India is the largest producer of lathyrus which is the third most important pulse crop after chickpea and pigeon pea. It is one of the most important climate resilient crops which thrive well not only in extreme weather and stressed condition but also in poor soil. It is a good source of dietary proteins and is used as food and fodder including improvement of soil fertility. The cultivation of this crop is neglected due to the presence of anti-nutritional compound β-N-oxalylamino-L-alanine (BOAA) or β-N- oxalyl-L-α, β-diaminopropionic acid (ODAP) which is considered as causative agent of the disease Lathyrism, the paralytic disorder. In recent past, many breeders started working to develop low-ODAP lines like Ratan, Prateek and BioL-212 etc. containing ~ 0.2% ODAP. Among different biotic factors, downy mildew (Peronospora lathi-palustris) poses a serious threat in case of water stagnation during winter rains. On the other hand, introgression of disease resistance gene leading to increase yield has also been initiated in grasspea using marker assisted selection. Therefore, it is necessary to develop trait linked molecular markers for marker assisted breeding for developing new Lathyrus varieties. The present work was aimed to screen out the segregants, from F2 and F3 population derived from two different cross combination, Berhampore local x Mahateora and Mahateora x BioL-212 attributed with high grain yield coupled with zero/negligible ODAP content and resistance to downy mildew resistance. In the present study, the segregating population (F2 and F3) developed from two sets of crosses (Mahateora x BIO L-212 and Berhampore Local x Mahateora) were characterized. Simple Sequence Repeats (SSR) marker-based assessment was done for parents and segregating populations in search of trait associated marker and to deduce molecular diversity. A total of 120 SSR markers including 30 EST-SSR were used for molecular diversity and screening for association with ODAP and /or Downey mildew. All morphometric traits were statistically significant in segregating populations derived from both the parental crosses. Based on the mean seed yield plant-1, the F2 segregants MBiF2-1, MBiF2-2 MBiF2-4 and three F3 Segregants MBiF3-43, MBiF3-54, and MBiF3-52 from Mahateora x BioL-212 as well as from Berhampore Local x Mahateora, F2 segregants BLMF2-23, BLMF2-37 and BLMF2-50 and F3 segregants BLMF3-46, BLMF3-143 and BLMF3-100 were found very promising. Low ODAP content (≤0.1%) was found in BLMF3- 32, BLMF3-78, BLMF3-8, BLMF3-186, BLMF3-39, BLMF3-46 and BLMF3-211. With respect to the percent disease incidence (PDI), the identified resistant segregants to Downy mildew were BLMF3-128 (2%), BLMF3-8 (4%), BLMF3-36, (12%) BLMF3-39 (12%), BLMF3-195 (14%) and BLMF3-100 (18%). The PDI was highest in BLMF3-38 (44%). Based on highest seed yield/plant (>20 g), low ODAP (≤0.1%) and PDI (<12%), the most promising segregants identified were BLMF3-39, BLMF3100, BLMF3211, BLMF3168. The seed yield/plant were found to be significantly and positively correlated at both genotypic and phenotypic level with Plant height, number of branches plant-1, number of pods plant-1, pod length, number of internodes plant-1 and number of seeds pod-1. The Regression model identified number of pod plant-1, 100 seed weight, number of seeds pod-1 and pod length in F2 of the crosses where as in the F3 population of Berhampore Local x Mahateora, number of pods plant-1, number of seeds pod-1, number of internodes plant-1, 100 seed weight, plant height and number of branches plant-1 were considered as the better explanatory variables. No significant differences between GCV and PCV were found for most of the traits, indicating lesser influences of environmental factors. High heritability coupled with high Genetic advance over mean for plant height, number of branches plant-1, number of pods plant-1, and seed yield plant-1 were recorded to be used for indirect phenotypic selection. Multivariate analysis based on morphometric parameters from the segregants from Mahateora x Bio L-212 viz, MBiF2-49, 2, 4, 55, 46, 20, 11, 19, 52, 33, 25 and MBiF3-52; from Berhampore Local X Mahateora the segregants BLMF2-27, 56, 30, 37, 50, 59 and, BLMF3-128, 143, 46, 211, 100, 168 and 39 were found as the diverged genotypes. Molecular diversity based on SSR genotyping formed 7 distinct groups where Cluster V was found to be the largest with 15 cluster members. Based on higher Polymorphic Information Content (PIC) and Marker Index (MI) value, the SSRs namely c39279_g1_i12, c46049_g2_i1, c34810_g1_i1, c35787_g1_i1, c43114_g1_i1, c46949_g2_i1, c13442_gi_i1 and S-168 could be used in molecular diversity analysis in lathyrus for future breeding programme. From the study, the EST-SSR marker c31994_g1_i1 and c35336_g1_i4 were found putatively associated with low ODAP content from both F2 and F3 population. In the F3 population c39249_g1_i1 and S-159 showed association with downy mildew resistance.