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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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  • ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF GENETIC MALE STERILE GENOTYPES IN COTTON (Gossypium Sp.)
    (University of Agricultural Sciences GKVK, Banglore, 2005-06-16) BHARATI MUDARADDI; B. M. KHADHI
    MOLECULAR CHARACTERIZATION OF GENETIC MALE STERILE GENOTYPES IN COTTON {Gossypiiim Sp.) 2004 Bharati Mudaraddi Major Advisor B.M.Khadi ABSTRACT Cotton is the most important textile fibre and knowledge on genetic relatedness among the advanced lines is essential for crop improvement. The genetic diversity analysis of QMS genotypes through molecular markers will be useful in diversifying the genotypes for creation of hybrid combination. Application of molecular markers is an interesting alternative for selecting male sterile plants. Twenty near isogenic diploid CMS lines were screened using 119 random decamer primers of which 82 markers were found to polymorphic with 61.7 per cent polymorphism. Out of 314 amplicons amplified, 187 were found to be polymorphic, with an average of 3.83 fragments per primer of which 2.28 were polymorphic. The similarity among genotypes ranged from 70 to 98 per cent. Genetic diversity analysis among fourteen near isogenic tetraploid GMS lines was carried out using 88 random decamer primers. Out of 310 fragments amplified, 190 were found to be polymorphic with an average of 5.44 fragments per primer, 3.33 fragments per primer were polymorphic. OPY primers were found to be highly polymorphic. Presence of genetic diversity was evidenced by genetic similarity indices (0.76 - 0.98). The sterile and fertile plants of different genotypes made independent clusters indicating their divergence. RAPD markers are used as a tool for estimating genetic diversity and can be used on continuing basis to document the available variability in the cotton germplasm. Since G. hirsutum and G.arboreum groups have been improved independently, these form separate clusters, depicting enormous variation among them despite having same genome. Markers OPB04 and 0PZ14 showed genetic diversity between fertile and sterile plants of all the diploid GMS genotypes. Similarly the marker OPB04 also showed genetic diversity within fertile and sterile plants of the tetraploid GMS genotypes. Hence, they can be considered as putative markers for linkage studies and identification of male sterile and fertile plants.