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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Pathology × Author: BASAVARAJ, S. × Start date: 2012 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    SURVEY, MOLECULAR DETECTION AND PARTIAL CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS (CMV) AND BANANA BRACT MOSAIC VIRUS (BBrMV) ISOLATES INFECTING BANANA IN KARNATAKA
    (University of Agricultural Sciences, Bangalore, 2014-09-12) BASAVARAJ, S.; Rangaswamy, K. T.
    The use of tissue culture planting material has helped to a greater extent to overcome the problems of viral diseases. However, the genetic variant associated with tissue culture plants has limited their use for raising the crop. Eighty five tissue cultured and sucker propagated banana gardens were surveyed for banana viruses and tissue culture variants. The mean incidence of virus diseases was high (3.78%) in gardens established using suckers compared to tissue culture established gardens (1.47%). The average incidence of somaclonal variants was 2.95 % in tissue cultured gardens. Out of 5532 samples diagnosed only 0.7% of the samples were found positive for viruses. Out of 3572, samples tested for genetic purity using ISSR markers, only 0.1% samples were found to not true to type. Novel, rapid and cost effective Loop mediated isothermal amplification (LAMP) based techniques were revalidated for detection of BBTV, BSV, CMV and BBrMV. Colorimetric indicator dyes like HNB, Calcein, SYBR Green I and thiazole orange successfully differentiated healthy and virus infected samples after amplification, thus colorimetric detection was standardised for detection of banana viruses. Out of 25 ISSR markers validated for their suitability to detect the most common tissue culture variants only three ISSR markers detected the variant with chimeric leaves. However, failed to detect other types of variants observed during survey. RT-PCR amplification of the CMV and BBrMV CP genes using gene specific primers resulted in the amplification of ~650 bp and ~1062 bp products respectively. Phylogenetic analysis of banana CMV and BBrMV isolates under present study based on both nucleotide and amino acid sequences of CP revealed that the CMV isolate belongs to subgroup IB and BBrMV isolate belongs to BBrMV Karnataka group.