Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

20072
Reset filters
Subject: Plant Pathology × Author: ASWATHANARAYANA, D.S × Start date: 1961 × Type: Thesis × Thesis Type: Ph.D ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF JATROPHA MOSAIC VIRUS
    (University of Agricultural Sciences GKVK, Banglore, 2007-06-06) ASWATHANARAYANA, D.S; K. V. KESHAVA, MURTHY
    No Abstract
  • Thumbnail Image
    ThesisItemOpen Access
    BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF JATROPHA MOSAIC VIRUS
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2007-06-06) ASWATHANARAYANA, D.S; KESHAVA MUTHRY, K.V
    Euphorbiaceae. The bark of the plant yields a dark blue dye, which is used for colouring cloths, fishing nets etc. Jatropha is affected by many pests and diseases. Mosaic disease caused by begomovirus is the most important among the diseases. The causal virus of the mosaic disease was characterized by symptomatology, vector transmission, EM, and by partial sequencing DNA-A component of the virus genome. Mosaic infected Jatropha plants exhibit symptoms of light chlorotic specks lareas , crinkling, blistering of leaves and severe stunting of plants. Flower abscission and malformation of fruits are observed on plants infected prior to flowering. The disease was found to occur in all the Jatropha growing areas of Karnataka state with the incidence ranging from 2.08 to 46.66 per cent. The disease incidence was lowest in Mysore district. Hassan district recorded the highest incidence. Analysis of the whitefly B. tabaci samples for B biotype using molecular technique revealed the prevalence of B biotype in all the areas. However , the vector population was relatively low on Jatropha (3-61plant). The disease was successfully transmitted through whiteflies B. tabaci, grafting and dodder (Cuscuta subinclusa), but not through seeds. The B biotype whitefly required less acquisition access period (AAP), inoculation access period (lAP) and number of whiteflies to transmit JMV when compared to indigenous whiteflies. Hundred per cent transmission of virus was achieved with 5 B biotype whiteflies as compared to 10 indigenous whiteflies. Jatropha mosaic virus was detected in the vectors whitefly B. tabaci, and Dodder (C. subinclusa) by PCR tests using Deng primers which primed PCR products of size ~500 bp specific to begomoviruses. The virus was detected in whitefly after separation from virus source up to 8 days (until their death) with decrease in the virus concentration indicating a persistent circulative virus- vector relationship. Jatropha Mosaic Virus was successfully transmitted to the plant species viz , Nicotiana tabaccum cv. Samsun, N. benthamiana, N. tabaccum, Euphorbia geniculata including Jatropha curcas and J gossypifolia. Electronmicroscopic observation of partially purified preparations from JMV infected leaf tissues revealed the association of geminate virus particles measuring 15-18 X 30 nm. Phylogenetic analysis of core CP nucleotide sequences of 575bp grouped JMV (B) and JMV (D) in a separate cluster next to cassava mosaic virus from India and Sri Lanka. They were 94 per cent similar to each other and shared highest nucleotide identity (93.9 %) with both ICMV (Tri) and SLCV (Ker) isolates. JMV from Puerto Rico and Jamica grouped separately from JMV from India, and shared 72.8 to 75.2 per cent core nucleotide identities. On the basis of symptomatology, particle morphology, Nucleotide sequence similarity with other begomovirus sequences, the causal agent of mosaic disease on Jatropha is identified as distinct Gemini virus transmitted by whitefly B. tabaci.