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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Biotechnology × End date: 2009 × Start date: 2006 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    CLONING AND EXPRESSION OF STREET RABIES VIRUS GLYCOPROTEIN GENE IN YEAST (Pichia pastoris)
    (University of Agricultural Sciences GKVK, Bangalore, 2009-05-16) NAGESHA, S. N.; RAMANJINI GOWDA, P. H.
    Rabies is a preventable infectious viral disease of mammals, most often transmitted through the bite of rabid animals and causes significant number of annual deaths in rural areas of Asia and Africa. Immunization with rabies vaccine is the most effective means of preventing rabies virus infection. As the presently available vaccine are expensive and involves risk of handling live virus hence inexpensive, safe and efficacious vaccines are urgently needed to address the infectious viral diseases. The yeast, Pichia pastoris, acts as an ideal system for expression of heterologous genes, as the post translational modifications like glycosylation, protein folding successfully occurs in the yeast and which secretes out the heterologous protein into media which makes protein purification simple. The glycoprotein of rabies virus is most antigenic and immunogenic determinant present in rabies virion and can serve as effective target for development of vaccine. Hence the street rabies glycoprotein gene (1.6 Kbp) was cloned into pBKS+ plasmid vector which was characterized by PCR, restriction digestion and by sequencing, further the rabies glycoprotein gene was subcloned into yeast transfer vector (pPICZαA) for expression. Linearised recombinant vector containing glycoprotein gene was introduced into yeast (Pichia pastoris, GS115). The recombinant yeast clones were confirmed by PCR analysis. The recombinant protein expressed in yeast using methanol at 0.5% (v/v) and the expression of glycoprotein gene product was confirmed by SDS-PAGE, the 70kDa protein confirmed the antigen specificity by western blot and ELISA. The expressed recombinant rabies glycoprotein could be used as vaccine after the immunization trials in animals.