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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Biotechnology × Author: RENUKA × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    MOLECULAR AND SEROLOGICAL DIVERSITY OF MUNG BEAN YELLOW MOSAIC VIRUS (MYMV) REPLICATION ASSOCIATION GENE.
    (University of Agricultural Sciences, Bangalore, 2018-08-01) RENUKA; ANITHA, PETER
    Mung Bean Yellow Mosaic Virus (MYMV) is an important virus causing the most devastating disease in mung bean. As the replication association gene (Rep) is involved in viral replication, site specific nicking, DNA binding, ligation, and helicase activity the knowledge of Rep gene sequences would be useful in efforts to know the genetic relationships among MYMV isolates and management of disease. In the present study an attempt was made to clone the Rep gene from MYMV and to identify the diversity of MYMV isolates from India and global isolates originating from different geographical region, the results of sequencing and bioinformatics analysis revealed that, MYMV isolates from eastern, southern and western India were highly heterogeneous in Rep gene length varied from 1080-1092 nucleotides and 359-363 amino acids. Maximum homogeneity at nucleotide and amino acid level was in northern (99 % and 98%) isolates, followed by eastern (95 % and 97 %), western (93 % and 95 %), Sothern (83 % and 98 %) and central (85 % and 95 %) isolates. All north Indian isolates were clearly separated from isolates of other geographical regions and formed a major group in phylogenetic trees and the clustering pattern did not correlate well with their geographical regions. The present study suggests that location specific strategy may have to be developed to manage MYMV. Along with these, serological studies in validation of polyclonal antibodies showed that its effectiveness in detecting virus at primary antibody dilutions of 1:500.
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR AND SEROLOGICAL DIVERSITY OF MUNG BEAN YELLOW MOSAIC VIRUS (MYMV) REPLICATION ASSOCIATION GENE.
    (University of Agricultural Sciences, Bangalore, 2018-08-01) RENUKA; ANITHA, PETER
    Mung Bean Yellow Mosaic Virus (MYMV) is an important virus causing the most devastating disease in mung bean. As the replication association gene (Rep) is involved in viral replication, site specific nicking, DNA binding, ligation, and helicase activity the knowledge of Rep gene sequences would be useful in efforts to know the genetic relationships among MYMV isolates and management of disease. In the present study an attempt was made to clone the Rep gene from MYMV and to identify the diversity of MYMV isolates from India and global isolates originating from different geographical region, the results of sequencing and bioinformatics analysis revealed that, MYMV isolates from eastern, southern and western India were highly heterogeneous in Rep gene length varied from 1080-1092 nucleotides and 359-363 amino acids. Maximum homogeneity at nucleotide and amino acid level was in northern (99 % and 98%) isolates, followed by eastern (95 % and 97 %), western (93 % and 95 %), Sothern (83 % and 98 %) and central (85 % and 95 %) isolates. All north Indian isolates were clearly separated from isolates of other geographical regions and formed a major group in phylogenetic trees and the clustering pattern did not correlate well with their geo-graphical regions. The present study suggests that location specific strategy may have to be developed to manage MYMV. Along with these, serological studies in validation of polyclonal antibodies showed that its effectiveness in detecting virus at primary antibody dilutions of 1:500.