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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Biotechnology × Author: NGUYEN PHUONG, THUY × Start date: 2006 ×
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    ThesisItemOpen Access
    MARKER ASSISTED SELECTION FOR ENHANCED ZINC CONTENT IN RICE
    (University of Agricultural Sciences, Bengaluru, 2018-08-20) NGUYEN PHUONG, THUY; SHASHIDHAR, H. E.
    A study was carried out using F3 and F4 progeny from a cross between ‘Gopaldoddiga’ and ‘ARB6’ cultivars. Analysis of variance for the traits evaluated showed highly significant differences (P < 0.001) among the genotypes for all traits. High estimates of GCV and PCV were observed for total number of tillers, number of productive tillers, biomass per plant, grain yield per plant and harvest index. GCV and PCV estimates were moderate for day to 50 % flowering, plant height, panicle length, 100 grains weight and brown grain Zn. PCV values were only slightly higher than GCV values under investigation for all traits. High estimates of broad sense heritability along with high GAM was observed for all the characters except GAM of plant height in F3 generation. Presence of positive relationship between grain yield and brown rice Zn concentration implies these traits can be used as a selection criterion from F4 generation onwards. To understand expression in Zn content at the sequence level, genes were identified as members of the ZIP family were chosen to design Zn transporter specific primers. Among twenty primers used three primers viz, OsZIP2-1, OsZIP3-2, OsZIP4-4 showed strong positive association with brown grain Zinc content. Brown rice Zn content and grain yield were high for hybrid progenies namely, GA 247-12-101, GA 247-12-141, GA 247-12-984, GA 240-450-35, GA 240-450-58, GA 240-450-21, GA 287-621-48, GA 287-63-172, GA 287-63-245, GA 287- 63-25, GA 214-132-20, GA 214-132-51, GA 214-132-136, GA 214-132-26 and GA 214- 132-279, and therefore can be recommended for planting under suitable aerobic conditions. These zinc enriched high yielding lines can be effectively utilized in rice biofortification programs across the globe.
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF T4 TRANSGENIC TOBACCO WITH HEPATITIS – B GENE
    (University of Agricultural Sciences GKVK, Bangalore, 40865) NGUYEN PHUONG, THUY; RAMANJINI GOWDA, P. H
    Hepatitis B is a potentially life-threatening liver infection caused by the HBV and is a major global health problem. The worldwide problem of HBV infection eventually led to the development of a vaccine. Immunization with Hepatitis B vaccine is the most effective means of preventing Hepatitis B virus infection and its consequences. As the available recombinant vaccine is expensive, cheaper and improved vaccines are urgently needed to address the global scourge of infectious diseases. Plants are a potential source of HbsAg, in addition, a plant based HBsAg expression system makes possible as an oral immunization strategy by simply feeding the plant products. The primary means of transformation is the Agrobacterium mediated gene transfer which has provided a reliable means of creating transformants in a wide variety of species and also can express a wide variety of pharmaceutically important products including recombinant vaccines. The present investigation lays emphasis on study of integration and stability of the recombinant protein expressed in Tobacco plants. Restriction digestion analysis of the gene construct pHB118 with restriction enzymes EcoRI and BamHI yielded two separate bands of 9.7kb and 3.6kb size. The leaf explants of tobacco were transformed with Hepatitis B surface antigen gene along with npt-II as an antibiotic selection marker gene. The presences of HBsAg gene in putative transformants were confirmed by PCR analysis. All the putatively transformed tobacco plants showed the presence of 900 bp band in the PCR analysis. The crude protein obtained from the transformed tobacco plants were tested by SDS-PAGE for presence of 24 kDa protein, western-blot and ELISA confirmed the antigen specificity and immunogenic nature of the Hepatitis B surface antigen. The T4 generation seeds obtained from the transgenic tobacco plants were tested for the germination in the presence of kanamycin. It was observed that the segregation ratio was 3:1 indicating Mendelian inheritance. The growth parameters of T4 generation transgenic and control tobacco plants showed there is no major variation in growth between transgenic and control plants.