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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Biotechnology × Author: BHASKARAREDDY, K. S. × End date: 2008 × Start date: 2000 ×
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    ThesisItemOpen Access
    DEVELOPMENT OF SEEDLESS GRAPE HYBRIDS THROUGH EMBRYO RESCUE AND CONFIRMATION OF HYBRIDITY BY MOLECULAR MARKERS
    (University of Agricultural Sciences GKVK, Bangalore, 2008-08-08) BHASKARAREDDY, K. S.; SAHIJRAM, LEELA.
    A study on development of seedless grape hybrids through embryo rescue and confirmation of hybridity by molecular markers was carried out with the objective of rescuing embryos in crosses involving ‘Thompson seedless’ as the female parent and four downy mildew resistant lines (SV12309, SV23501, SV12364, SV18315) as the pollen parents, to identify hybrids resistant to downy mildew (fungal) pathogen Plasmopara viticola, and, to develop molecular markers for grape hybrids so-generated. Using conventional breeding procedures, it is not possible to obtain hybrid progeny in grape crosses involving seedless (‘stenospermocarpic’) female parents. Such crosses can, however, be salvaged using sequential embryo rescue and hybrids can be obtained. For culturing ovules, berries of various crosses were collected at 8, 9, 10 and 11 Weeks Post Pollination (WPP). Initiation of growth and maintenance of ovules was done on Emershad and Ramming semi-solid medium supplemented with Casein hydrosylate (50mg/l)+ Myo-inositol (50mg/l)+ LCysteine (211.6 mg/l)+ Morpholino Ethane Sulphonic acid (1000mg/l). At 8 weeks from in vitro incubation, embryos were excised from ovules and subcultured on semi-solid Woody Plant Medium (WPM) supplemented with benzyl adenine (BA) @ 0.25mg/l. In four to eight weeks, the embryos started germinating and subsequently developed into full-fledged plantlets in liquid medium. Hybrid plantlets were then transferred to polybags containing Soilrite ( 75% Peat moss + 25% Perlite). A total of 122 hybrid plantlets were recovered from 955 embryos sub-cultured (which were excised from 1698 cultured ovules). The true hybrid nature of these hybrids was confirmed by using ISSR (UBC-807, UBC-817, UBC-825, UBC-828) markers.