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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Plant Biochemistry × Author: ASHISH, MARATHE × Start date: 2016 × Type: Thesis ×
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    ThesisItemOpen Access
    PURIFICATION AND CHARACTERIZATION OF AN ANTIVIRAL PROTEIN FROM THE SILKWORM Bombyx mori L.
    (University of Agricultural Sciences GKVK, Bangalore, 41113) ASHISH, MARATHE; ANITHA, PETER
    Sericulture is an important agrobased industry. India stands second next to China among the silk producing countries of the world. Silkworm is susceptible to fungal, bacterial, viral and protozoan diseases. The Bombyx mori nucleopolyhedrosis virus (BmNPV) is the most harmful virus in the sericulture industry. The Antiviral Red Fluorescent Protein from the silkworm was purified from the digestive juice and the excreta of the silkworm. The red fluorescent protein (RFP) was isolated and purified by ammonium sulfate saturation, organic solvent precipitation and gel filtration chromatography on sephadex G-100 column. The fractions that fluoresce red in U.V were used for further analysis. The protein from the digestive juice and excreta were subjected to Native and Denaturating PAGE. The electrophoregram of the purified protein from the digestive in native PAGE has shown two bands and the SDS – PAGE pattern for the RFP from the gut fluid revealed the presence of four different bands. The electrophoregram of the purified protein form the feacal matter in native PAGE showed a single band which coincided with the first band in the protein from the gut juice. The SDS-PAGE pattern revealed two bands of which one was found to be around 28 kDa and the other band was smaller than 14.4 kDa. Dot Blot was carried out to detect the specificity between the antigen and the antibody. ELISA was also done to fix the titre of the antigen and antibody. The titre obtained was 1:25 of antigen and 1:2000 of primary and secondary antibody dilutions.