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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Forestry × Author: FASIHA × End date: 2023 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON ENDOSPERM DERIVED TRIPLOID CLONE OF NEEM (Azadirachta indica A. Juss.) FOR MORPHOGENIC RESPONSE IN VITRO
    (University of Agricultural Sciences, Bangalore, 2018-08-01) FASIHA; MAHADEVA MURTHY, M.
    World over, the primary interest on Neem research is centered around documenting the active principles and working on the methods to enhance the principles for commercial interests. The research done at the Plant Tissue Culture Laboratory, UAS, GKVK, Bengaluru in collaboration with C-Camp, NCBS, Bengaluru had yielded a triploid clone from the endosperm culture in vitro. This particular clone on UHPLC-MS had shown a hundred-fold increase in Azadirachtin content. The present research was aimed at finding out efficient in vitro interventions for plant regeneration from of the triploid clone. Repeated trials over a period of 5 to 6 months from leaflet and rachis explants failed to provide aseptic cultures. In order to obtain aseptic cultures, realizing that the explant material of this only available clone is very limited to only one plant, A novel methodology of forcing new vegetative buds by pruning of shoots was done. The culture of newly emerged shoots which just turned green gave aseptic cultures. A maximum amount of callus (1.7) was recorded (by scoring) on MS media supplemented with the combination of 5.0 mg L-1 NAA+0.5 mg L-1 BAP in leaf explant. Further, upon subculture, onto the MS media containing same concentration of growth regulators, an increment in callus growth (3.89) was obtained. Best morphogenic response in terms of maximum number of shoots (5.67) from callus was obtained upon culture of the callus onto the media containing 1 mg L-1 BAP and the maximum number of (1.6) adventitious roots were produced in callus cultured on the MS basal medium.