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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Entomology × Author: VENU, H S × End date: 2022 × Start date: 2020 × Type: Thesis ×
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    SCREENING OF Bacillus thuringiensis ISOLATES AGAINST GREATER WAX MOTH (Galleria mellonella L.) INFESTING Apis cerana AND THEIR MOLECULAR CHARACTERIZATION
    (University of Agricultural Sciences, Bangalore, 2022-12-23) VENU, H S; SHYLESHA, A N
    Greater waxmoth, Galleria mellonella L. is an important pest of honeybees and remained as nightmare for beekeeping farmers. In the present study, the effectiveness of different strains of Bacillus thuringiensis were evaluated against Galleria mellonella. The biocontrol potential of NBAIR-BtGa gave pest mortality rate of 68 per cent followed by NBAIR-VGa1f (60%), and standard HD-1 (52%), with respective LC50 values of 257.03, 350.26 and 407.38 μg/ml. Whole genome sequencing of genomic DNA and plasmid of NBAIR-BtGa, NBAIR-BtVGa1 and NBAIR-BtVGa2 samples resulted in the identification of a size of 5.96, 0.81, 0.97 and 0.89 Mb, respectively consisting of 6109, 779, 971 and 903 protein coding genes, respectively. Through Cry gene profiling, a total of five Cry genes (Cry1Da1, Cry2Af1, Cry1Cb1, Cry1Ac18, and Cry1a14), two Cry genes (Cry1a14 and Cry2Ab), and two Cry genes (Cry2Aa1 and Cry1Ac5), respectively, were detected in the plasmids of the NBAIR-BtGa, NBAIR-BtVGa1, and NBAIRBtVGa2. Other insecticidal genes viz., Vip3Bb2, Cyt genes, Hemolytic enterotoxin, Zwittermycin A resistance protein, Hemolysin protein, Serine protein kinase, Bacillolysin, DEAD BOX helicase was also observed in this study have toxicity towards lepidopteran pests. The biosafety studies of Bacillus thuringiensis isolates on honeybees showed that the isolates were 70-80 per cent and 90 per cent safer to adults and larvae of honeybees, respectively. Bacillus thuringiensis strains were subjected to bioassay, biosafety, and whole genome sequencing (WGS), which demonstrated the strain’s efficacy as a biocontrol agent will facilitate the development of bioformulations for the management of Galleria mellonella.