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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Crop Physiology × Author: B. H, Rajashekar Reddy × Start date: 2012 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Assessment of variability for phytic acid content in mini-core germplasm lines of finger millet (Eleusine coracana (L.) Gaertn.) and development of RNAi Lines for low phytic acid content
    (University of Agricultural Sciences, Bengaluru, 2013-12-13) B. H, Rajashekar Reddy; A. G, Shankar
    Phytic acid is generally regarded as the primary storage form of phosphorous, from human nutrition perspective, Phytic acid is an efficient chelator of nutritionally important mineral cations such as Ca, Fe, Mg, Zn etc. ,and these nutrients are not bioavailable for monogastric animals including humans. For these reasons, in the last two decades one of the biofortification approaches has been the isolation and development of ‘low phytate’ (lpa) cereals and legume genotypes with the aim to improve bioavailability of already existing nutrients in food crops. Finger millet is considered as one of the important minor cereal due to high nutritional value with highest calcium content among staples foods. Initially in our study to access the variability for phytic acid content 124 finger millet germplasm lines were screened for phytic acid and inorganic phosphorous content. Analysis of Variance for mean values revealed the significant differences among the accessions for phytic acid and Inorganic phosphorous. Further estimation of genetic parameters showed variations for the trait were mostly due to genetic factors and Principal Component Analysis also revealed Phytic acid is component contributing to the variability across germplasm accessions. The rate limiting enzyme in phytic acid biosynthesis is myo-inositol 3-phosphate synthase (MIPS) and altering MIPS expression in developing seeds may lead to a valuable reduction in phytic acid content. In this direction, full length MIPS gene (1818 bp) from immature embryo of finger millet was cloned. In-silico analysis of EcMIPS gene sequence showed longest coding region of 1533 bp which encodes 510 amino acid residues with NAD Binding 5 super family domain which is characteristic feature of MIPS enzymes. Further, RNA interference (RNAi) technology was used for down regulation of MIPS. An efficient siRNA forming region from EcMIPS without any off- targets were identified and these regions were used to develop RNAi constructs both under constitutive and endosperm specific promoter. For plant transformation Agrobacterium mediated gene transfer technology was followed. Characterization of putative transformants with reduced phytic acid content need to be performed.