Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

20072
Reset filters
Subject: Biotechnology × Author: ROOPA, K.P × End date: 2019 ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
  • Thumbnail Image
    ThesisItemOpen Access
    "Biochemical, Histochemical and Molecular Characterization of Trichoderma harzianum isolated from different agroclimatic zones of Karnataka and its interaction with medicinal plant (Nigella sativa L)".
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2007-05-21) ROOPA, K.P; SURESH, C.K
    The investigation was carried out to study the biochemical, histochemical and molecular characterization of Trichoderma harzianum that occur in the soils of the ten different agroclimatic zones of Karnataka. One Trichoderma spp. was isolated from each agroclimatic zones usingPotato dextrose agar medium and purified. These isolates werecharacterized as T harzianum strains. Pure culture of the ten Trichoderma harzianum representing each zone along with a reference culture was maintained in the Department of Biotechnology, were separately grown in Potato dextrose broth medium. Molecular diversity of these isolates was characterized by using SDS-PAGE and RAPD markers. SDS-PAGE analysis revealed thatRmvalue of the bands ranged from 0.0246 and 0.995. In RAPD a total of 125 bands were scored out of which 100 bands were found to be polymorphic (80.00%). Statistical analysis of SDS-PAGE andRAPD data enabled the classification ef lOT harzianum isolates in to two major groups. The protein profile data and RAPD banding pattern of these isolates closely separate isolates from differentzones. Apot culture experiment was carried out to study germination, growth, biochemical and histochemical parameters on the medicinal plant Nigella sativa under glass house conditions. Nigella sativa plants inoculated with the T harzianum strains recorded increased germination, plant height, number of branches and biomass compared to uninoculated plants. Biochemical parameters include chlorophyll content, nitrogen, phosphorous, total sugars, reducing sugars and total soluble proteins. Among the 10 strains inoculated, the T harzianum strain isolated fromthe zone6 (SDT) recorded the highest plant growth, biochemical and histochemical parameters, which is followed by the strains isolated from other zones. The least biomass, biochemical and histochemical parameters were recorded in uninoculated plants.