Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

20072
Reset filters
Subject: Biotechnology × Author: JAYAPADMA, P. N. × Start date: 1970 × Type: Thesis × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    TRANSFORMATION STUDIES IN CHILLI (Capsicum annuum L.)
    (University of Agricultural Sciences GKVK, Banglore, 2007-03-06) JAYAPADMA, P. N.; SUMANGALA, BHAT
    Chilli is a vegetable and spice crop of worldwide importance. It is used in various foods as a natural colorant. Despite the fact that other closely related members of the Solanaceae family are easily transformed by genetic engineering, capsicum still remains recalcitrant to in vitro manipulation. This research is an attempt to establish suitable regeneration and transformation protocol for two local cultivars of chilli viz., Byadgi Dabbi and Sankeshwar local. In order to standardize regeneration protocol through somatic embryogenesis, whole cotyledon explants were cultured on MS medium supplemented with different levels of Thidiazuron (TDZ) (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mg/J) and MS with 3 mg/I TDZ (87.8%) was found better. Complete plantlet formation was occurred on MS with 3 mg/I ABA or 2 mg/I IBA. In order to standardize protocol for multiple shoot bud elongation, whole cotyledon explants were cultured on MS with higher levels of BAP (8.0, 10.0, 12.0 mg/I) or MS with 10 mg/I BAP + 2 mg/I GA3. Percent multiple shoot bud induction was high on medium with 10 mg/I BAP (90.3%) and number of shootbuds per explant was high on 8 mg/I BAP. These shoot buds were cultured on low levels of BAP (0.0 1, 0.05, 0.1, 0.5 mg/I) and combination of 0.05 mg/J BAP with two levels of lAA (0.05 and 0 .1 mg/I). 0.05 mg/I BAP along with 0.05 rng/I lAA was found better for elongation. Rooting of the elongated shoots were obtained on 2 mg/I IBA. In order to standardize transformation protocol, shoot tip explants (4-5 mm) were co-cultivated for two days with Agrobacterium strain EHA105 harbouring pBinBt3 having crylAc gene and nptII marker linked to the CaMV35S promoter and nos terminator. These co-cultivated explants were cultured on medium with 2 mg/l IBA containing 300 mg/I cefotaxime and 100 mg/I kanamycin. Plants surviving on this medium were tested through PCR using nptII specific primers. Of the 20 plants tested four plants were positive for nptII amplification. These plants were further tested for the expression of crylAc gene using Bt-xpress kit and only three were positive
  • Thumbnail Image
    ThesisItemOpen Access
    TRANSFO MATION STUDIES IN CHILLI (Capsicum annuum L.)
    (University of Agricultural Sciences GKVK, Banglore, 2007-03-06) JAYAPADMA, P. N.; SUMANGALA, BHAT
    N0 Abstract