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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Agricultural Microbiology × Author: SANTHOSH, G. P. × Start date: 2007 × Type: Thesis ×
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    ThesisItemOpen Access
    ANTIMICROBIAL PEPTIDES OF INVERTEBRATES
    (University of Agricultural Sciences GKVK, Banglore, 2008-07-01) SANTHOSH, G. P.; G.P. BRAHMPRAKASH
    The present study focussed on isolation, screening and characterization of antimicrobial peptides from invertebrates. Sixteen invertebrates were screened for their antimicrobial activity against six microorganisms. Body fluid extracts of immune activated invertebrates (with overnight grown culture of Escherichia coli) exhibited higher antimicrobial activity compared to unchallenged ones. Higher invertebrates exhibited good antimicrobial activity even without immune activation. Body fluid extracts of selected invertebrates when subjected to 60° C, 100°C (boiling temperature of water) and 121° C (autoclaving temperature) for 15 min, extracts showed thermostability by exhibiting antimicrobial activity even with increased temperature levels. Antimicrobial activity of body fluid extracts was reflected on its minimum inhibitory concentration. Body fluid extract of selected invertebrates exhibiting higher antimicrobial activity was found to be active even at lower concentrations. Most active concentration for inhibition of test organism was observed when assayed without dilution. Total protein content of body fluid extracts of invertebrates decreased after being challenged compared to unchallenged ones. However there was variation in carbohydrate contents of challenged and unchallenged body fluid extracts. Protein fractions in both unchallenged and challenged body fluid extracts of invertebrates were similar, except in case of Samia cynthia ricini and Hirudo medicinalis. Minimum inhibitory concentrations of purified fractions of body fluid extracts of selected invertebrates showed that, most effective concentration was, when they were used without dilution. Though activity spectrum of body fluid extracts of invertebrates was wide, purified peptides of selected extracts exhibited activity only against Escherichia coli.
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    ThesisItemOpen Access
    ANTIMICROBIAL PEPTIDES OF INVERTEBRATES
    (University of Agricultural Sciences GKVK, Bangalore, 2008-06-22) SANTHOSH, G. P.; BRAHMAPRAKASH, G.P.
    The present study focussed on isolation, screening and characterization of antimicrobial peptides from invertebrates. Sixteen invertebrates were screened for their antimicrobial activity against six microorganisms. Body fluid extracts of immune activated invertebrates (with overnight grown culture of Escherichia coli) exhibited higher antimicrobial activity compared to unchallenged ones. Higher invertebrates exhibited good antimicrobial activity even without immune activation. Body fluid extracts of selected invertebrates when subjected to 60o C, 100o C (boiling temperature of water) and 121o C (autoclaving temperature) for 15 min, extracts showed thermo-stability by exhibiting antimicrobial activity even with increased temperature levels. Antimicrobial activity of body fluid extracts was reflected on its minimum inhibitory concentration. Body fluid extract of selected invertebrates exhibiting higher antimicrobial activity was found to be active even at lower concentrations. Most active concentration for inhibition of test organism was observed when assayed without dilution. Total protein content of body fluid extracts of invertebrates decreased after being challenged compared to unchallenged ones. However there was variation in carbohydrate contents of challenged and unchallenged body fluid extracts. Protein fractions in both unchallenged and challenged body fluid extracts of invertebrates were similar, except in case of Samia cynthia ricini and Hirudo medicinalis. Minimum inhibitory concentrations of purified fractions of body fluid extracts of selected invertebrates showed that, most effective concentration was, when they were used without dilution. Though activity spectrum of body fluid extracts of invertebrates was wide, purified peptides of selected extracts exhibited activity only against Escherichia coli.