Thumbnail Image

University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

Browse

20071
Reset filters
Subject: Agricultural Microbiology × Author: ANUJA GEORGE × End date: 2007 × Start date: 2007 × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION, SCREENING AND SELECTION OF EFFICIENT CHLORPYRIPHOS DEGRADING MICROORGANISMS
    (University of Agricultural Sciences GKVK, Banglore, 2007-08-30) ANUJA GEORGE; K. S. JAGADEESH
    The focus of the present study was to develop bacteria capable of bioremediation of chlorpyriphos contaminated soil. This was attempted by isolating 28 bacterial isolates from chlorpyriphos enriched soil samples and screening for biodegradation of the pesticide. Of the screened bacterial isolates, 13 grew on mineral salts medium containing 100 ppm chlorpyriphos as the sole carbon source. There was only a slight increase in the release of chloride when the medium was supplemented with additional carbon sources such as succinic acid, glucose and maltose (@ 1 g/1). The most promising chlorpyriphos degrading bacteria were Pseudomonas sp. JA 15 and Enterobacter sp. JA 8 which degraded the pesticide by 98 and 93 per cent respectively in 7 days. Calcium alginateimmobilized Pseudomonas JA15 strain resulted in complete degradation of chlorpyriphos by 100 hours. Thin layer chromatographic analysis of all the isolates revealed appearance of an unknown metabolite having Rf value in the range 0.15-0.19. The enzyme involved in chlorpyriphos degradation by Pseudomonas JA15 isolate was found to be extracellular. These efficient strains were selected for the bioremediation of chlorpyriphos contaminated soil. The bioaugmentation of the strains alone and in combination to the contaminated soil resulted in higher degradation rate than was observed in uninoculated soils. The dual inoculation resulted in 97 per cent degradation in two weeks. The phytotoxic effects of the pesticide on germination and growth of cowpea were counteracted by the strains. The population of chlorpyriphos degrading bacteria in the polluted soil was found to increase throughout the period of investigation due to breakdown of the pesticide. There was also a significant increase in the population of total bacteria, fungi and actinomycetes in the rhizosphere due to inoculation with any of these strains. And, cowpea root exudates were found to support growth and multiplication of Pseudomonas JA 15 strain.