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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Author: Neena Kumari × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDIES ON INCIDENCE OF AEROMONAS SPP. IN READY-TO-EAT FOODS, BEVERAGES AND WATER FROM DIFFERENT TOURIST PLACES OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2018) Neena Kumari; Panda, A.K.
    The present study was designed with the aim to determine the incidence of Aeromonas species in ready-to-eat foods, beverages and water from different tourist places of Himachal Pradesh. A total of 220 samples; RTE milk products (n=80), RTE meat/fish products (n=40), beverages (n=40) and water (n=60) were collected from 12 different tourist places. In addition, 50 stool samples from hospitalized patients were also screened. Aeromonas isolates were characterized by cultural and biochemical methods and reconfirmed by amplifying 16S rRNA (953 bp) through polymerase chain reaction (PCR). Enterotoxin genes alt (encoding heat-labile cytotonic enterotoxin), ast (encoding heat-stable cytotonic enterotoxin) and act/hlyA/aer (encoding cytotoxic/β-haemolysin/aerolysin) complex present in characterized Aeromonas isolates were detected by PCR. A total of 20 Aeromonas spp. isolates were recovered from tested samples; 16 from RTE foods, beverages, water and 4 from human stool samples. RTE milk products had highest level of contamination (n=12/80, 15%) followed by beverages (n=3/40, 7.5%) and water (n=1/60, 1.67%). These isolates were identified as A. hydrophila (n=7/20, 35%), A. sobria (n=6/20, 30%), A. schubertii (n=4/20, 20%) and A. veronii (n=3/20, 15%). All Aeromonas isolates carried alt and 25 per cent were positive for act/hlyA/aer complex. None of the isolates was carrying ast. High level of susceptibility was observed for antibiotic classes; nitrofurans, phenicols and tetracyclines followed by quinolones and aminoglycosides. However, all the isolates were resistant to ampicillin. Nine (45%) isolates were multidrug resistant. Highest number of MDR isolates were recovered from RTE milk products (25%) followed by stool samples (15%) and beverages (5%).