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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Author: KUMAR Sharma, Amit × Start date: 2008 × Type: Thesis ×
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    ThesisItemOpen Access
    INVESTIGATION ON FOLLICULAR DYNAMICS AND SEMEN CRYOPRESERVATION IN GOATS
    (CSKHPKV, Palampur, 2018-01-06) KUMAR Sharma, Amit; Sood, Pankaj
    ABSTRACT Goats are multipurpose animals. Two different breeds, namely Gaddi and Chegu, strengthen the socioeconomic status of a large percentage of rural population in Himachal Pradesh. Out of season breeding in Gaddi breed is known, but has not been proved scientifically. Genetic degradation in both Gaddi and Chegu breeds due to lack of males of superior germplasm is seriously restricting the benefits from goat rearing. Accordingly, in present study (May 2016 to December 2017) information on breeding practices adopted by regional Gaddi shepherds (n=25) was collected and out of season breeding was confirmed to occur in 80% Gaddi goats. To verify breeding during long days, experimental studies on ovarian follicular dynamics were undertaken during non-breeding (n=11) and breeding seasons (n=7) in the same Gaddi does raised under semi-intensive conditions. Follicular dynamics revealed active ovaries during both seasons as indicated by a preponderance of 3- and 4-follicular waves in 94.4% animals. The ovaries were more active during breeding than non-breeding season. It was reflected by higher daily number of follicles of different sizes/day (5.50±0.55 versus 3.15±0.67) and higher growth rate of dominant follicle (0.79±0.04 versus 0.65±0.03 mm/d). The most distinct feature was presence of relatively larger dominant follicle during non-breeding than breeding season (7.66±0.10 versus 6.85±0.24 mm) which, however, remained anovulatory and could be incriminated to a shorter interwave interval during breeding season (4.90±0.31 versus 5.66±0.28 d). To overcome the paucity of superior males, a cryopreserved semen bank was established using elite Gaddi (n=11; aged 2.16±0.36 years) and Chegu (n=8; aged 1.98±0.34 years) bucks. The semen cryopreservation protocol was established using 74 ejaculates from Gaddi bucks. Removal of seminal plasma and incorporation of 10% egg yolk and 6% glycerol in the extender provided best post thaw semen in the study. The standardized protocol was eventually used to cryopreserve 106 and 180 qualifying ejaculates, collected twice weekly, in Gaddi and Chegu, respectively. In terms of progressive motility, the sperms of Gaddi compared to Chegu were more resilient to cryoinjury as indicated by a relatively reduced per cent change of 52.03 versus 58.31 and accordingly significantly higher progressive motility of 35.18±0.87 versus 30.86±0.78, respectively. In terms of age, the sperms of young Gaddi bucks than adult bucks were more cryosensitive, while such disparity was missing in Chegu. The semen quality improved with a reduction in temperature, while there was no such relation with photoperiod (sunshine hours) in both the breeds. The average fertility of cryopreserved semen generated in present study used as double straw coupled with 4 µg GnRH administration at induced estrus resulted in a fertility percentage of 41.25% in Gaddi (confirmed pregnancy in 33 out of 80) and 67.50% (non return rates in 27 out of 40). In conclusion, (i) the discrepancy in out of season breeding in Gaddi does at farmers versus experimental conditions could be due to variation in management practices (ii) the ovaries are active, but with anovulatory waves during non-breeding season (iii) removal of seminal plasma, use of 10% egg yolk and 6% glycerol provides best semen quality from Gaddi and Chegu bucks with acceptable fertility results (iv) Gaddi sperms are less cryosensitive (v) it is temperature instead of photoperiod that could have varying effects on semen quality of Gaddi and Chegu bucks.