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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Veterinary Pharmacology and Toxicology × Author: Kanika × Start date: 2014 × Type: Thesis ×
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    ThesisItemOpen Access
    ANTIOXIDANT AND IMMUNOMODULATORY ACTIVITY OF GLYCYRRHIZA GLABRA AND BAUHINIA VARIEGATA IN RATS
    (CSKHPKV, Palampur, 2014-07-18) Kanika; Varshneya, C.
    The present investigation was undertaken to study the antioxidant and immunomodulatory action of Glycyrrhiza glabra root and Bauhinia variegata stem bark extract. The fine powders of roots of Glycyrrhiza glabra and stem bark of Bauhinia variegata were subjected to methanolic, aquamethanolic and aqueous extraction and recovery of the extracts was 15.5, 19 and 10.24%, respectively, in case of Glycyrrhiza glabra and 30, 9.65 and 16.66 %, respectively for Bauhinia variegata. ABTS quenching activity (expressed as IC50 values) was 0.114 mg ml-1, 0.193 mg ml-1 and 0.709 mg ml-1 for methanolic, aqua-methanolic and aqueous extracts of Glycyrrhiza glabra, respectively whereas it was 0.276 mg ml-1, 0.302 mg ml-1 and 0.757 mg ml-1 for Bauhinia variegata extracts, respectively. DPPH radical quenching activity (expressed as IC50 values) was 0.159 mg ml-1, 0.571 mg ml-1 and 0.552 mg ml-1 for methanolic, aqua-methanolic and aqueous extracts of Glycyrrhiza glabra , respectively whereas it was 0.258 mg ml-1, 0.395 mg ml-1 and 0.410 mg ml-1 for Bauhinia variegata extracts, respectively. These results suggested that methanolic extract had better quenching activity for reactive radicals. The in vivo antioxidant activity was studied in rats (administred extract of both plants at dose of 100 mg kg-1 b.w. p.o. for 30 days) by evaluating lipid peroxidation, reduced glutathione and catalase in liver, kidney and erythrocytes of male Wistar rats (130-190g). Potassium dichromate at dose rate of 30mg kg-1 b.w. orally for 30days was used to induce oxidative stress in rats. The studies revealed a significant (P<0.05) decrease in lipid peroxidation in liver, kidney and erythrocytes and a significant (P<0.05) increase in reduced glutathione level in the liver and kidney of Bauhinia variegata and Glycyrrhiza glabra treated rats in comparison to oxidative stress induced rats. Further immunomodulatory studies were conducted in the rats immunocompromised with dexamethasone (5mg kg-1 b.w. interaperitoneally for 5 days). Dexamethasone caused immunosuppression characterized by significant lymphocytopenia.The studies further revealed a significant increase in footpad thickness of Glycyrrhiza glabra treated rats at 24 and 48 hours of challenge with 10 % SRBCs administered interadermally on sub planter region of footpad. There was a significant increase in the absolute lymphocyte count and haemagglutination titre in Glycyrrhiza glabra treated rats. On differential leucocyte count a significant lymphocytopenia observed in deaxamethasone treated rats. The histopathological evaluation of lymphoid tissue exhibited the enhanced lymphocyte proliferation in lymphoid follicles in Glycyrrhiza glabra and Bauhinia variegata treated rats. There was significant (P<0.05) increase in CD4+ receptor cell count in lymphocytes in Glycyrrhiza glabra treated rats in comparison to rats immunocompromised with dexamethasone. Further Glycyrrhhiza glabra had significantly (P<0.05) higher CD4+ cell count as compared to Bauhinia variegata treated rats and CD4+ cell count was comparable to placebo treated animals.The present studies revealed that Glycyrrhiza glabra has got a better antioxidant as well as immunomodulatory activity as compared to Bauhinia variegata.