Thumbnail Image

Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

Browse

20182
Reset filters
Subject: Plant Pathology × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: Ph.D ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    STUDIES ON GENE ACTION FOR SEED YIELD AND ITS RELATED TRAITS IN URDBEAN [Vigna mungo (L.) Hepper]
    (CSKHPKV, Palampur, 2018-09-18) Patial, Ranjana; Mittal, R.K.
    The present investigation entitled “Studies on gene action for seed yield and its related traits in urdbean [Vigna mungo (L.) Hepper]” was carried out at the Experimental Farm of the Department of Crop Improvement, CSK HPKV, Palampur to gather information on genetic architecture for seed yield and component traits in urdbean. The experimental material comprised of 81 triple test cross progenies developed by mating 27 lines with three testers viz., HPBU-111 (L1), Him Mash-1(L2) and F1 of HPBU-111and Him Mash-1 (L3). These F1’s along with their parents were raised in randomized block design with three replications during Kharif 2017. Data were recorded and analysed for 12 agro-morphological traits. Sufficient variability was observed in the TTC progenies for all the traits studied. Epistasis was found to be an integral part of genetic variation for all of the traits under study. ‘i’ type epistatic interaction estimates were significant for days to 50% flowering, days to 75% maturity, branches per plant, pods per plant, seed yield per plant, biological yield per plant, harvest index and 100 seed weight. Whereas, ‘j+l’ type interaction was significant for all the traits. Both additive and dominant components were significant for all the traits; where additive and dominance components were of almost equal magnitude for pod length, seeds per pod and 100 seed weight indicating the importance of both additive and dominance type of gene action and additive component being more pronounced for rest of the traits indicating the relative importance of fixable type of gene action in their inheritance. Combining ability estimates showed significant genetic variance in lines for all traits whereas testers had significant genetic variance for nine traits. Lines IC-436910, IC-413306, IC-IC-398973 and IC-343885 were found to be good general combiners for most of the traits. Crosses IC-343885 x HPBU-111 (G x G), IC-436910 x HPBU-111 (G x G), IC-413306 x Him Mash-1 (G x P) and IC-343943 x HPBU-111(P x G) were potential crosses on the basis of SCA estimates, mid parent heterosis, better parent heterosis and standard heterosis for seed yield and most of the traits. With regards to the Comparison of ТГС and L x T, the former is advantageous in providing an unambiguous test for the presence of epistasis, while the latter provides additional information, particularly with regard to the gca and sca effects and variances, helping breeders in the choice of better parents. Hence, both these designs should be applied together in order to have concrete information about the genetic architecture of economic traits in any crop.
  • Thumbnail Image
    ThesisItemOpen Access
    CHARACTERIZATION OF PEPPER MILD MOTTLE VIRUS STRAINS AND EVALUATION OF RESISTANCE IN CAPSICUM
    (CSKHPKV, Palampur, 2018-06) Nidhi Kumari; Sharma, P.N.
    Capsicum (Capsicum annuum L. var.grossum Sendt) is an important spice and vegetable crop being cultivated worldwide. More than 20 viruses are known to infect Capsicum spp. across the world and Pepper mild mottle virus (PMMoV), a member of Virgaviridae family and Tobamovirus genus is emerging as a great threat to the capsicum cultivation both in protected and open conditions in Himachal Pradesh (H.P.). The present study on PMMoV was undertaken to identify the pathotype of PMMoV prevalent in H.P., production of polyclonal antiserum against PMMoV-CP expressed in E. coli, evaluation of resistance against PMMoV in capsicum and identification of attenuated/mild strain if any. Surveys conducted to assess the prevalence of mild mottle disease showed wide occurrence of the disease and out of 97 samples collected during surveys, 54 showed the presence of PMMoV in DAS-ELISA with maximum percentage of positive samples from district Kullu (88.89%) followed by Mandi (78.57%) district. The presence of PMMoV was confirmed through RT-PCR using coat protein (CP) specific primers where positive samples yielded amplification of ~743 bp. Isolates were selected for varaiblity assays on differential varieties of capsicum and CP gene sequence analysis. All the isolates produced symptoms like mosaic, color variations, leaf cupping, vein banding on susceptible cultivar California Wonder. Based on the pathogenic reaction on differential varieties and amino acid sequence of CP gene, all the test isolates were grouped and identified as pathotype P12 which can overcome L+, L1 and L2 resistance alleles. For production of polyclonal antiserum, the PMMoV-CP was over-expressed in E. coli using IPTG at 1mM final concentration with overnight incubation in shaking incubator at 16oC which resulted in induction of target recombinant protein with molecular weight ~26kDa. The antiserum generated through out sourcing, evaluated for its sensitivity and specificity through Western blot and DAC-ELISA. In Western blot assay, the test antiserum reacted strongly both with PMMoV-CP in purified protein and native CP in crude sap from PMMoV infected pepper plants, whereas no reaction was observed with healthy plant sap. In DAC-ELISA antiserum dilution up to 1:1000 was capable of differentiating the PMMoV infected sample from healthy samples. The antiserum did not react with other capsicum viruses viz., Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), Pepper veinal mottle virus (PVMoV), Potato virus Y (PVY) and Tomato yellow leaf curl virus (TYLCV) antigen. Only two exotic capsicum accessions PI-159236 and PI-260429 were found resistant to PMMoV. None of the isolates showed the characteristics of attenuated/mild strain as all the isolates produced prominent symptoms on susceptible cv- California Wonder.