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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20143
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Subject: Plant Pathology × End date: 2014 × Start date: 2014 ×
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    ThesisItemOpen Access
    GENE ACTION FOR YIELD AND RELATED TRAITS IN SOYBEAN [Glycine max (L.) Merrill] AND DEVELOPMENT OF INTERSPECIFIC HYBRIDS INVOLVING WILD SPECIES
    (CSKHPKV Palampur, 2014-12) INDU BALA; Jai Dev
    The present investigation entitled “Gene action for yield and related traits in soybean [Glycine max (L.) Merrill] and development of interspecific hybrids involving wild species” was carried out at the Experimental Farm of the Department of Crop Improvement, CSK HPKV, Palampur to gather information on genetic architecture for seed yield and component traits in soybean and to introgress desirable genes from wild species to cultivated ones. The experimental material comprised of 54 triple test cross progenies derived by mating 18 lines with three testers, namely, Hara Soya (L1), Him Soya (L2) and their F1 (L3). This genetic material was evaluated in a randomized complete block design with three replications during kharif 2013. Epistasis was found to be an integral part of genetic variation for majority of the traits. Epistatic interaction for many traits was ‘j+l’ type except days to 50% flowering, days to 75% maturity, reproductive phase, petiole length, seed per pod, harvest index and 100 seed weight, whereas plant height, internode length, pods per plant and biological yield per plant carried ‘i’ type epistasis alongwith ‘j+l’ type. Additive component (D) was more pronounced than dominance component (H) for most of the traits. Both additive and dominance components were of almost equal magnitude for pod length indicating the importance of both additive and dominance type of gene action, whereas partial degree of dominance was noticed for majority of traits. The kind of genetic variance revealed from triple test cross can be exploited by intermating selected individuals in early segregating generations with delayed selection in later generations, diallel selective mating/ biparental mating or recurrent selection followed by pedigree method to exploit both additive and non-additive components alongwith epistasis. Lines Bragg, Shivalik and P9-2-2 were found to be good general combiners for most of the traits. The cross combinations, Bragg x Hara Soya, PK- 472 x Him Soya, DS-1213 x Hara Soya, H-330 x Hara Soya and H-330 x Him soya showed high per se performance, SCA effects, heterobeltiosis and economic heterosis for seed yield per plant and were rated as potential crosses for further improvement. Cross H-330 x Him Soya showed resistance against brown spot and bacterial pustule diseases. The interspecific hybrids developed involving Glycine max x G. soja, were true to type based on confirmation at the morphological, molecular and cytological level. Sufficient variability was found for all the traits studied in wide hybrids. Seed yield per plant showed significantly positive correlation with reproductive phase, pods per plant, pod length, petiole length, harvest index and 100-seed weight.
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    ThesisItemOpen Access
    ORGANIC MANAGEMENT OF BUCKEYE ROT OF TOMATO CAUSED BY PHYTOPHTHORA NICOTIANAE VAR PARASITICA (DASTUR) WATERHOUSE
    (CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur, 2014) KRISHAN, RAJ; PAUL, Y.S.
    Present investigations on “Organic management of buckeye rot of tomato caused by Phytophthora nicotianae var parasitica (Dastur) Waterhouse.” were conducted in the Department of Plant Pathology, CSKHPKV, Palampur during 2012-2014. P. nicotianae var parasitica was found to be associated with buckeye rot of tomato from all the locations in HP. PDA supported the best mycelial growth and sporangial formation of the pathogen. Out of 43 bacterial and fungal isolates from organic inputs and phyllosphere, one isolate KLCuB3 was most effective in the management of buckeye rot even if the fruits were injured before or after treatment. Complete inhibition of the pathogen growth was observed under in vitro conditions when organic inputs, crude extract of Eupatorium adenophorum, aqueous extract of marigold, urine of desi cow, pine oil were used. Linseed oil panchgavya formulation, was found superior and economical in the inhibition of the pathogen and disease management. Eupatorium, pine oil, panchgavya and DMA8 completely checked the disease whereas except sumona and T. viride, no commercial product completely managed the disease. As the fruit stage progressed, the susceptibility against the disease decreased. Three lines namely Ptom, BT18 and Palam Pride showed some level of resistance. No disease developed when soil was either sterilized or drenched with panchgavya, neemastra, jeevamrit, amritpani, ghaniri extract and akshwan. BTH enhanced resistance and delayed the appearance of the disease. Trichoderma koningii (DMA8) resulted in highest per cent inhibition. Six sprays of Eupatorium crude extract @ 0.2% at weekly intervals were found to be most effective in the management of buckeye rot followed by Trichoderma (DMA-8) and Ridomil 0.25%. Plastic mulch + soil treatment with DMA-8 proved more effective to manage the disease. Integration of seedling dip with pine oil with plastic mulch was not suitable as it reduced the plant population and increased the disease level.
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF NL-1N STRAIN OF BCMV INFECTING COMMON BEAN IN HIMACHAL PRADESH AND DEVELOPMENT OF DIAGNOSTIC KITS
    (CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur, 2014) ANURADHA; Sharma, P.N.
    Common bean (Phaseolus vulgaris L.), locally known as “Rajmash” one of the most widely grown grain legume crop grown around the world is vulnerable to the attack of wide range of plant pathogens. Among these bean mosaic disease caused by Bean common mosaic virus (BCMV), a member of family Potyviridae is most important to low yield contributing factor because of its regular occurrence and ubiquitous seed borne nature. In India, five strains of BCMV viz., NL-1, NL-1n, NL-4, NL-7 and NL-7n have been reported from north-western Himalayas, of which NL-1n is widely prevalent in the entire region. The present investigations on BCMV- NL-1n strain were undertaken to obtain complete genome sequence, relationship with different strains and to elucidate the presence of R-genes to find out durable sources of resistance. Typical symptoms produced were mosaic, blistering, downward leaf rolling, green vein banding, thickening of leaves, reduction of leaf lamina and leaf deformation which were more severe on seed borne infected plants as compared to sap inoculated. DAS-ELISA test and coat protein gene amplification confirmed the identity of virus as BCMV and reaction pattern on International differential set of bean varieties established the strain identity. This strain was separated from other BCMV strains on the basis of temperature sensitivity assay as it induced temperature insensitive necrosis on cv. Jubila that contains resistance gene (I, bc-1), thus differs from all other strains of BCMV and BCMNV. The genome size was 10.081 kb and contains an open reading frame that encoded a single polyprotein of 3222 amino acids which cleaved into ten proteins typical of Potyvirus. Sequence homology, multiple alignment and phylogenetic analysis on nucleotide and amino acid sequences showed that BCMV-NL-1n is more closely related to BCMV-NL-1 though clustered along with other strains and isolates of BCMV. HC-Pro region sequences based comparison among BCMV strains showed its potential for the discrimination of different strains, however, it still needs further validation involving more number of isolates. Under artificial inoculation conditions, 130 common bean accessions were found resistant to strain BCMV-NL-1n in a panel of 304 accessions comprising of diverse germplasm. BCMV resistance gene prediction based on marker assisted selection (MAS) approach, using tightly linked SCAR markers showed the presence of three resistance genes viz., I, bc-12 and bc-3 genes in 105, 53 and 22 common bean accessions found resistant in the present study. Five genotypes viz., IC-43567, EC-109731, EC-116178, EC-120636 and Improved Tender Green possessed all the three genes. The resistant sources identified against the prevalent strains can be exploited in resistance breeding programme.