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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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2004 - 200982010 - 20167
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Subject: Plant Breeding and Genetics × Start date: 2004 × Type: Thesis × Thesis Type: Ph.D ×
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Now showing 1 - 9 of 15
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    ThesisItemOpen Access
    Studies on induced mutagenesis in ricebean [vigna umbellata thunb. ohwi and ohashi]
    (CSKHPKV, Palampur, 2008) Patial, Madhu; Thakur, S.R.
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    ThesisItemOpen Access
    Genetic assessment of maize inbreds for the development of desirable hybrids
    (CSKHPKV, Palampur, 2007) Jenjiha, Jagdish Kumar; Sood, B.C.
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    ThesisItemOpen Access
    Studies on gene action for yield and its components in linseed (Linum usitatissimum L.)
    (CSKHPKV, Palampur, 2005) Sood, Salej; Kalia, N.R.
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    ThesisItemOpen Access
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Genetic analysis of yield components and net-assimilation rate in rice (Oryza sativa L.)
    (CSKHPKV, Palampur, 2004) Sanjeev Kumar; Singh, H.B.
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    ThesisItemOpen Access
    Genetic amelioration of local basmati rice through induced mutagenesis
    (CSKHPKV, Palampur, 2004) Uttam Chand; Katoch, P.C.
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    ThesisItemOpen Access
    TAGGING OF THE SPECIFIC GENOME AND CHROMOSOME(S) OF HEXAPLOID WHEAT TRIGGERING CHROMOSOME ELIMINATION IN WHEAT x IMPERATA CYLINDRICA SYSTEM OF DOUBLED HAPLOIDY BREEDING
    (CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur, 2013) KAILA, VINEETA; Chaudhary, H. K.
    The present research endeavour entitled as “tagging of the specific genome and chromosome(s) of hexaploid wheat triggering chromosome elimination in wheat x Imperata cylindrica system of doubled haploidy breeding” was undertaken at Molecular Cytogenetics & Tissue Culture Lab and Experimental Farm of the Department of Crop Improvement, CSK HP Agricultural University, Palampur, Himachal Pradesh, India during 2010- 11 and 2011- 12 with the objective of identifying the wheat genomes and chromosomes responsible for elimination of whole I. cylindrica chromosome complement in wheat x I. cylindrica hybrids during early embryogenesis. Upon hybridization of different species of wheat viz., Triticum monococcum, T. tauschii and T. durum with I. cylindrica, haploid embryos were recovered in T. tauschii and T. durum only which revealed that the D and B genomes of hexaploid wheat are triggering the paternal chromosome elimination process. The possibility of enhancing the efficiency of embryo formation via increasing the exogenous supply of 2,4-D was also exploited and 0.03 % and 0.04 % of 2,4-D concentration were found optimum for haploid induction in T. durum and T. tauschii, respectively. The induction of haploid embryos in these species was also confirmed by cross-sectional studies of fertilized ovules derived after their hybridization with I. cylindrica. For further resolution of the genetic control of paternal chromosome elimination in wheat x I. cylindrica crosses, cytogenetic stocks viz., complete set of monosomic and nullisomic lines derived from ‘Chinese Spring’ cultivar of hexaploid wheat and D genome substitution lines of tetraploid wheat cultivar ‘Langdon’ were crossed with I. cylindrica. Statistical analysis of embryo formation frequency in monosomics revealed the role of 4D, 1D, 6B and 6D chromosomes as the absence of these chromosomes resulted in reduction of haploid embryo formation frequency from control as well as mean. These results were also supported by data obtained from nullisomics and substitution lines. Morphological characterization of monosomic series was carried out on the basis of 24 traits to identify visual markers for differentiation of aneuploids from disomic plants. Eight morphological descriptors were identified for differentiation of nullisomics, that is, seed size, plant height, spike length, number of tillers, florets per spike, seeds per spike, days to flowering and 100 seed weight. Whereas, two descriptors were identified in case of monosomics viz., spike shape for 5A and days to flowering for 2B monosomic.
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    ThesisItemOpen Access
    Imperata cylindrica - MEDIATED INDUCTION OF HAPLOIDS IN VARIOUS CEREALS AND ENHANCEMENT OF THE SYSTEM IN WHEAT THROUGH COLCHICINE MANIPULATIONS
    (CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur, 2010) TAYENG, TISU; Chaudhary, H K
    The research endeavour entitled ―Imperata cylindrica- mediated induction of haploids in various cereals and enhancement of the system in wheat through colchicine manipulations‖ was executed in the Department of Crop Improvement, CSK HPKV, Palampur during the years 2007- 08 to 2009- 10 with the mission to induce haploids in wheat and other cereals using Imperata cylindrica as a pollen source and efficiency enhancement of the chromosome elimination-mediated system of doubled haploidy breeding in wheat through various colchicine manipulations at in vivo and in vitro level. For the execution of wide hybridization, five cereals viz., wheat, rice, maize, barley and oat involving various promising lines/ genotypes were utilized and for colchicine manipulation experiments at in vitro and in vivo level, various formulation and combination of time, duration and colchicine were formulated and executed in DH 100 x DH 40 derived F1 hybrid of bread wheat. The results obtained in the present investigation in respect of wide hybridization of different cereals with I. cylindrica showed that I. cylindrica was an efficient and effective pollen source for haploid induction in bread wheat and durum wheat. Pseudo seeds and embryo like structures were recovered in barley and rice upon hybridization with I. cylindrica. However, green plants could not be regenerated in both the cases. Maize and oat didn‘t respond well but there can be a ray of hope for the induction of haploids in these cereals also if more refinement and manipulation is done in hybridization techniques and culture protocols. In the in vivo colchicine manipulation experiment, colchicine concentration ranging from 2000 ppm to 10,000 ppm at 48, 72 and 96 hours after pollination have yielded response to chromosome doubling. Application of injection of single dose of 2000 ppm colchicine concentration at 48 hours of pollination has given most stable results in doubling the chromosome number. Hence, it can be recommended as the best dose for achieving fruitful results and save time and energy spent on this exercise through earlier techniques. In the in vitro colchicine manipulation experiment, the colchicine concentration ranging from 300 ppm to 3000 ppm of 48, 72 and 96 hours of treatment induced doubling of the chromosomes. The outcome of the present endeavour can open new vistas for enhancing the efficiency of DH breeding in wheat and other cereals with enhanced precision and efficiency.