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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20201
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Subject: Plant Biotechnology × Author: Ankita × Start date: 2006 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON CRYOPRESERVATION AND REGENERATION OF IN VITRO RAISED CULTURES OF PICRORHIZA KURROA
    (CSKHPKV. Palampur, 2020-09-28) Ankita; Shashi, Bhushan
    Picrorhiza kurroa is an endangered Himalayan perennial herb and important ingredient of traditional medicinal systems of Asian region. It’s medicinal value primarily depends upon presence of picrosides in its roots. These bioactives are used for preparation of hepatoprotective phytopharmaceuticals. This herb is uprooted from wild for extraction of bioactive ingredients, which is a major reason for its depletion from natural habitat. Considering the high demand and low supply of P. kurroa population, efforts for its conservation through biotechnological tools is urgently required. In current research, cryopreservation protocol of high picroside producing cell lines of Picrorhiza kurroa were developed. P. kurroa shoot cultures incepted with 0.50 mg/L of Kn. Highest in vitro average shoot number was observed at 0.50 mg/L Kn strengthened MS medium with 3.18 shoots per shoot tip whereas 4.29 cm shoot length with 11.46 average number of leaves recorded on MS basal medium. Highest callus induction (100%) was found on MS medium augmented with 0.0125 mg/L IBA + 0.125 mg/L TDZ from leaf and root explants. Calli multiplied on the same medium with 6.89 fold increase in fresh weight. Leaf and root explants of in vivo and in vitro grown plants were analysed for identification of high metabolite producing cell lines. Among in vivo leaf and root explants, highest P-I content (5.65 %) was found in leaves whereas highest P-II content (8.37 %) was found in roots. In case of in vitro leaves and roots, leaves contained higher P-I (1.160 %) & P-II (0.280 %) content. Callus derived from leaves also showed highest total picroside content i.e 0.2 per cent, therefore, leaf derived callus was used for cryopreservation. Among all the cryopreservation methods used i.e encapsulation-vitrification, vitrification and desiccation, only encapsulation-vitrification showed 60 per cent regeneration at 15 minutes of dehydration time with PVS2 solution. Encapsulation-vitrification protocol would be useful for ex-situ conservation of P. kurroa callus cultures.