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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Microbiology × Start date: 2005 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    EVALUATION OF FUNCTIONAL TRAITS OF INDIGENOUS BACTERIAL PROBIOTICS OF NORTH-WESTERN HIMALAYAS
    (Department of Microbiology, COBS CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 2016-11) SHARMA, SAKSHI; Kanwar, S.S.
    ABSTRACT Eleven indigenous probiotic bacteria obtained from traditional fermented foods of North- Western Himalayas were screened for their functional attributes including antagonistic activity against strict anaerobic pathogens viz., Clostridium perfringens, Bacteroides fragilis and Peptostreptococcus anaerobius. The growth of indigenous probiotics in selected prebiotics was maximum in lactulose followed by fructooligosaccharide and inulin. Lactic and short chain fatty acids viz., acetic, propionic and butyric were the principle metabolites observed after fermentation of prebiotics by probiotics as detected by UPLC-MS analysis. The growth of anaerobic pathogens in prebiotics was almost negligible, thereby indicating their safe use without any ill effect to the host. The antagonistic activity against strict anaerobic pathogens was analysed in cell free crude filtrates of probiotics and expressed in terms of specific activity. Maximum specific activity in crude filtrate was observed with AdF10 (L.plantarum) against C.perfringens followed by P. anaerobius and B.fragilis. The antagonistic activity present in crude filtrate was purified by ammonium sulphate precipitation and gel filtration chromatography yielding 17.88 fold increase in purification. The specific activity of crude filtrate on purification was increased from 105.26 AUmg-1protein to 1882.35AUmg-1protein against C.perfringens. The antagonistic activity was primarily mediated by proteinaceous compound(s) as the activity was lost after treatment with proteolytic enzymes. The characterization of partially purified fractions by Tris-Tricine SDS–PAGE analysis revealed a wide range of proteins with molecular mass in the range of 3.5-100 kDa. The adherence of eleven indigenous probiotics was evaluated using Caco-2 and HT-29 cells as in vitro models. Among all probiotics, AdF10 (L. plantarum) was found to be the most adhesive to HT-29 and Caco-2 cell lines with % adhesion of 12.88±0.63 and 9.55±0.76, respectively, which was statistically at par with reference strain L.rhamnosus GG. The three inhibition assays were employed to determine the adherence inhibition ability of probioics against anaerobic pathogens. Maximum inhibition in adherence was observed with exclusion assay as compared to competition and displacement assays. The interaction of different prebiotics with pathogens resulted in reduction of adherence of pathogens to tested cell lines. The crude supernatants obtained from indigenous probiotics were effective in checking the proliferation of transformed cell lines as detected by MTT assay indicating the utility of probiotics as biotherapeutic agents.