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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20172
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Subject: Genetics and Plant Breeding × Author: Singh, Kritika × End date: 2019 × Start date: 2010 ×
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    ThesisItemOpen Access
    IDENTIFICATION OF AN EFFICIENT CHROMOSOME DOUBLING AGENT FOR ENHANCING DOUBLED HAPLOID PRODUCTION EFFICIENCY IN WHEAT × Imperata cylindrica DERIVED WHEAT HAPLOIDS AT in vivo AND in vitro LEVEL
    (CSKHPKV, Palampur, 2017-12-18) Singh, Kritika; Chaudhary, H.K.
    The research endeavour entitled ―Identification of an efficient chromosome doubling agent for enhancing doubled haploid production efficiency in wheat × Imperata cylindrica derived wheat haploids at in vivo and in vitro level‖ was executed in the Department of Crop Improvement, CSK HPKV, Palampur during the years 2015-16 to 2016-17 with the goal to determine the relative efficiency of colchicine and other potential chemicals for chromosome doubling in I. cylindrica- mediated haploids at in vitro and in vivo level and identify most potential and economically viable chromosome doubling agent capable of inducing significantly high frequency of doubled haploids in wheat. The material for the present investigation comprised of six wheat F1s (DH-100 × DH-5, DH-5 × DH-100, DH-40 × DH-5, DH-5 × DH-40, DH-65 × DH-5 and DH-5 × DH-65) which were utilized as female parents whereas Imperata cylindrica was utilized as pollen source. During rabi 2015-16 and 2016-17, wheat x I. cylindrica (from last week of March to April) hybridization was carried out followed by treatment of haploids at in vitro and in vivo levels with six chromosome doubling agents namely, colchicine, pronamide, amiprophos methyl, caffeine, oryzalin and trifluralin. To assess the efficiency of different chromosome doubling agents at in vitro level, the embryos of each genotype were cultured in media consisting each of chemicals at three different concentrations and four different durations. At in vivo level, the plants were treated with each of the six chemicals at 3-4 leaf stage for three concentrations and four durations. The response of various chromosome doubling agents applied for different concentration and varied duration revealed significant difference for various haploid induction and doubled haploidy parameters. The statistical analysis of data generated over two years regarding various parameters showed that chromosome doubling was reported at 1500 ppm and 2000 ppm colchicine for 72 hours, pronamide 2 ppm and 3 ppm for 96 hours, amiprophos methyl 5 μM for 96 hours and oryzalin 10 μM for 48 hours and 96 hours at in vitro and 750 ppm and 1500 ppm colchicne for 4 hours & pronamide 2 ppm and 4 ppm for 8 hours and 3 ppm for 16 hours at in vivo level. The chromosome doubling agents which induced doubled haploid production exhibited similar efficiency at in vitro and in vivo level. Pronamide was found to be most economically viable chemical for DH production. DH-100 × DH-5 and DH-5 × DH-100 performed better than other genotypes with respect to various haploid induction as well as doubled haploidy parameters. Use of different chromosome doubling agents to find most potential chemical at in vitro and in vivo level for induction of doubled haploids has opened new vistas in bread wheat improvement programme.
  • Thumbnail Image
    ThesisItemOpen Access
    IDENTIFICATION OF AN EFFICIENT CHROMOSOME DOUBLING AGENT FOR ENHANCING DOUBLED HAPLOID PRODUCTION EFFICIENCY IN WHEAT × Imperata cylindrica DERIVED WHEAT HAPLOIDS AT in vivo AND in vitro LEVEL
    (CSKHPKV, Palampur, 2017-12-18) Singh, Kritika; Chaudhary, H.K.
    The research endeavour entitled ―Identification of an efficient chromosome doubling agent for enhancing doubled haploid production efficiency in wheat × Imperata cylindrica derived wheat haploids at in vivo and in vitro level‖ was executed in the Department of Crop Improvement, CSK HPKV, Palampur during the years 2015-16 to 2016-17 with the goal to determine the relative efficiency of colchicine and other potential chemicals for chromosome doubling in I. cylindrica- mediated haploids at in vitro and in vivo level and identify most potential and economically viable chromosome doubling agent capable of inducing significantly high frequency of doubled haploids in wheat. The material for the present investigation comprised of six wheat F1s (DH-100 × DH-5, DH-5 × DH-100, DH-40 × DH-5, DH-5 × DH-40, DH-65 × DH-5 and DH-5 × DH-65) which were utilized as female parents whereas Imperata cylindrica was utilized as pollen source. During rabi 2015-16 and 2016-17, wheat x I. cylindrica (from last week of March to April) hybridization was carried out followed by treatment of haploids at in vitro and in vivo levels with six chromosome doubling agents namely, colchicine, pronamide, amiprophos methyl, caffeine, oryzalin and trifluralin. To assess the efficiency of different chromosome doubling agents at in vitro level, the embryos of each genotype were cultured in media consisting each of chemicals at three different concentrations and four different durations. At in vivo level, the plants were treated with each of the six chemicals at 3-4 leaf stage for three concentrations and four durations. The response of various chromosome doubling agents applied for different concentration and varied duration revealed significant difference for various haploid induction and doubled haploidy parameters. The statistical analysis of data generated over two years regarding various parameters showed that chromosome doubling was reported at 1500 ppm and 2000 ppm colchicine for 72 hours, pronamide 2 ppm and 3 ppm for 96 hours, amiprophos methyl 5 μM for 96 hours and oryzalin 10 μM for 48 hours and 96 hours at in vitro and 750 ppm and 1500 ppm colchicne for 4 hours & pronamide 2 ppm and 4 ppm for 8 hours and 3 ppm for 16 hours at in vivo level. The chromosome doubling agents which induced doubled haploid production exhibited similar efficiency at in vitro and in vivo level. Pronamide was found to be most economically viable chemical for DH production. DH-100 × DH-5 and DH-5 × DH-100 performed better than other genotypes with respect to various haploid induction as well as doubled haploidy parameters. Use of different chromosome doubling agents to find most potential chemical at in vitro and in vivo level for induction of doubled haploids has opened new vistas in bread wheat improvement programme.