Loading...
Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur
Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country.
The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh.
Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.
Browse
10 results
Search Results
ThesisItem Open Access MOLECULAR GENETIC CHARACTERIZATION OF CERTAIN GROWTH RELATED GENES AND ANALYSE THEIR ASSOCIATION WITH SOME GROWTH TRAITS IN GADDI GOATS.(CSKHPKV, Palampur, 2019-07-22) Gitanjali; Thakur, Y.P.This study was undertaken for molecular genetic characterization of IGF-1 gene and four fragments of GH gene in Gaddi goats and analyse their association with growth traits. Blood samples from 63 genetically unrelated animals of Gaddi goat breed were taken from the migratory flocks under AICRP and Gaddi goat unit of CSKHPKV, Palampur and subjected to DNA isolation using Phenol Chloroform extraction technique. 363, 422, 116, 389 and 181 bp amplicons were generated on amplification of target loci of IGF-1 and GH gene. IGF-1 gene, GH1 and GH2 fragments were analysed using PCR-RFLP (HaeIII RE) that revealed two variants (AB and BB) for IGF-1 and GH2 whereas, three variants (AB, BB and AA) were revealed for GH1 fragment. GH3 and GH4 fragments were subjected to PCR-SSCP that detected three genotypes (AB, BB and AA) for GH3 however, GH4 was found to be monomorphic. Significant association (P≤ 0.05) was detected for GH1 with 9- month body weight, GH2 with 9 and 12 months’ heart girth and GH3 with 6-month body weight, body height and body length, respectively. Allele frequencies for ‘A’ allele were 0.25, 0.53, 0.62 and 0.67 and 0.75, 0.47, 0.38 and 0.33 for ‘B’ allele. The PIC values revealed median level of polymorphism in studied population. From the present study, it was concluded that SNPs and their association with some body measurements may be useful in selecting goats for higher growth traits.ThesisItem Open Access MOLECULAR GENETIC STUDIES OF SOME GENES ASSOCIATED WITH FECUNDITY, GROWTH AND DISEASE RESISTANCE IN NATIVE SHEEP AND GOAT BREEDS OF HIMACHAL PRADESH(CSKHPKV, Palampur, 2018-07) Sankhyan, Varun; Thakur, Y.P.Sheep and goat husbandry is a predominant livestock activity in harsh climatic regions of the country. The present study was undertaken with the objectives to study genetic polymorphism of some genes related with fecundity, growth and disease resistance in native sheep and goat breeds of Himachal Pradesh. Genomic DNA was isolated from 63, 68, 197 and 73 animals belonging to Gaddi sheep, Rampur Bushair sheep, Gaddi goats and Chegu goats, respectively. Screening of different breeds for established ovine fecundity genes revealed that out of 8 point mutations screened for GDF9, BMP5, and BMPR1B genes, 2 point mutations, (G4-GDF9) and (G7-GDF9) were detected in Gaddi sheep and Rampur-Bushair sheep. PCR-RFLP screening of KISS1 gene in Gaddi goats revealed, frequency of A and T alleles as 0.43 and 0.57 and T allele was associated with litter size in Gaddi goats. PCR-RFLP of PRLR gene in Gaddi goats, revealed SNP (T92C). The SSCP analysis for Growth Hormone (GH) gene revealed 12 different SSCP patterns. Different variants of GH gene fragments revealed significant association with body weights and body measurements. PCR-RFLP analysis of Insulin like Growth Factor-1 (IGF-1) and Myostatin gene revealed SNP (G264C) and SNP (A70G), respectively. The AB heterozygote genotype of myostatin gene demonstrated significantly higher body weight at 3 and 12 month and body girth at 9 month of age. DRB3 gene revealed 2 alleles and 3 restriction digestion profiles and 4 alleles and 7 restriction digestion profiles for PstI and HaeIII digestion respectively. PCR-RFLP analysis of DQB gene using HaeIII revealed two alleles A and B with frequency ranging from 0.45 to 0.62 and 0.38 to 0.55 in different breeds. The present study generated genotypic data for genes related with fecundity, growth and disease resistance for native sheep and goat breeds of Himachal Pradesh, for which the genotypic characterization information was limited. The generated information could be a valuable input for conservation and future genetic improvement of the breeds.ThesisItem Open Access Studies on genetic and non genetic variations of angora rabbits reared in Kangra valley of Himachal Pradesh(CSKHPKV, Palampur, 2010) Sarma, Loopamudra; Katoch, SanjeetThesisItem Open Access STUDIES ON GENETIC AND NON GENETIC VARIATIONS OF ANGORA RABBITS REARED IN KANGRA VALLEY OF HIMACHAL PRADESH(CSKHPKV, Palampur, 2010-07) SHARMA, Loopamudra; Katoch, SanjeetABSTRACT This study was conducted to evaluate the phenotypic profiles, genetic and non genetic effects on wool production and reproduction traits in 588 and 361 German Angora rabbits maintained at Angora Rabbit Breeding Farm, Kandwari, Kangra (Himachal Pradesh) from 2000 to 2009 for reproduction traits and from 2000 to 2007 for wool production traits. The data was analyzed by Harvey’s least squares method of fitting constants. The overall least squares means were 4.62 1.64 k, 30.39 0.58 d, 58.77 35.10, 169.77 30.38, 181.00 21.30, 184.73 23.49, 594.22 67.13, 591.82 193.67 and 1185.96 202.96 g respectively for litter size at birth, gestation period, wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Year and season of kindling significantly affected litter size at birth, wool yield at first, third, fourth clip and first year yield. Gestation period, wool yield at second clip, second year and cumulative yield were affected by year. Heritability estimates were 0.09, 0.29 0.030, 0, 0.312 0.021, 0.27 0.017, 0.412 0.028 and 0.116 0.013 respectively for wool yield at first, second, third, fourth clip, first year, second year and cumulative yield. Genetic correlations of wool yield at second clip with second year and cumulative yield, wool yield at fourth clip with second year and cumulative yield and first year with second year and cumulative yield were negative. Wool yield at first clip with cumulative yield, second with third clip and third with fourth clip, first year, second year and cumulative yield were zero. Wool yield at second clip with fourth clip had the highest genetic correlation (1.226 0.398). Phenotypic correlations of second clip with second year and third clip with second year yield were negative. Second year and cumulative yield had the highest significant phenotypic correlation (0.942). Winter appeared to be the most favorable season for breeding of Angora rabbits. Improvement can be achieved with proper selection and improved management practices. Pure breeding stock of Angora rabbits can be introduced to maintain superior genotypes and variability.ThesisItem Open Access GENETIC CHARACTERIZATION OF HILL CATTLE OF HIMACHAL PRADESH USING MOLECULAR MARKERS(CSKHPKV, Palampur, 2008) Kumar Mahajan, Kailash; Kaila, O.P.ThesisItem Open Access MICROSATELLITE BASED MOLECULAR GENETIC CHARACTERIZATION OF CHEGU–PASHMINA GOAT BREED(CSKHPKV, Palampur, 2015) SHARMA, ASHISH; Thakur, Y.P.ThesisItem Open Access GENETIC STUDIES ON SOME ECONOMIC TRAITS IN RAMBOUILLET x GADDI (HIMTAL MERINO) CROSSBRED SHEEP UNDER SUBTEMPERATE CONDITIONS(CSKHPKV, Palampur, 2016-02) Thakur, Shalini; Thakur, Y.P.ABSTRACT A crossbred sheep population locally named as “Himtal Merino” with 75% Rambouillet inheritance have been evolved at Govt. Sheep Breeding Farm, Tal (Hamirpur) by crossing of Gaddi ewes with Rambouillet rams. Data on 1204 crossbred lambs for growth and wool production, 669 ewes for reproductive and 370 sheep for lifetime performance traits, progeny of 28 sires, were analysed by LSMLMW method using a fixed effect model to study mean performances and effect of some genetic and non-genetic factors on performance traits. The variance and co- variance components for genetic parameters were estimated by single trait animal model using WOMBAT software. The overall least squares means were 3.26±0.06, 16.11±0.03, 21.82±0.06 and 26.29±0.12 kg for lamb‟s weight at birth, 3, 6 and 12 months of age respectively, 141.44±0.18 g/day for pre weaning growth rate and 63.68±0.34 g/day during 3-6 months of age, 24.53±0.45 g/day during 6-12 months age and 36.64±0.38 g/day over 3-12 months of age post weaning body weight gain. The means were higher for HTM than R x G and R x RG lambs. Higher growth rate was achieved during pre weaning than post weaning stages. The mean age at first breeding and first lambing were 924.25±0.78 and 1070.51±0.48 days respectively. For wool production traits, the means for age at first shearing (AFS) was 184.62±0.14 days, shearing interval as 180.29±0.07 days, first and second annual greasy fleece were 1823.30±1.59 g and 2163.83±1.83 g respectively, second annual yield being higher than first. The mean longevity was 1210.72±26.39 days with productive life span of 945.84±26.39 days. The heritability for growth traits was low to medium being 0.19±0.05, 0.14±0.04, 0.20±0.05 and 0.06±0.03 for birth, 3, 6 and 12 months of age body weights. High heritability (0.62±0.01) was estimated for pre weaning body weight gain. For post weaning body weight gains, heritability were 0.14±0.04 for 3-6 months, 0.15± 0.04 for 6-12 months and 0.10±0.04 for 3-12 months of age. The heritability for various reproductive traits were low to moderate with estimates of 0.22±0.02, 0.02±0.10, 0.08±0.11, 0.07±0.10, 0.21±0.04, 0.31±0.06, 0.16±0.03, 0.18±0.05 for age at first breeding, age at first lambing, first lambing interval, second lambing interval, first service period, second service period, first gestation period and second gestation period respectively. For wool production traits, low heritability of 0.19±0.07 and 0.18±0.06 was estimated for age at first shearing and shearing interval and low to moderate heritability of 0.21±0.06, 0.14±0.04, 0.24±0.11, 0.23±0.09, 0.31±0.08 and 0.29±0.07 for fleece yield at first, second, third and fourth clips, first and second annual fleece yields respectively. Most genetic and phenotypic correlations among growth traits were positive and high. Positive genetic correlations were observed among various wool production traits and growth traits and wool production traits indicated scope for selection on basis of early expressed growth traits.ThesisItem Open Access GENETIC STUDIES ON INHERITANCE OF PART LACTATION MILK YIELDS IN RED SINDHI X JERSEY CROSSBRED COWS(CSKHPKV, Palampur, 2010-06) Verma, Nishant; Thakur, Y.P.ABSTRACT Records on 136 Red Sindhi x Jersey crossbred cows, maintained at Dairy Farm, Himachal Pradesh Krishi Vishva Vidyalaya, Palampur for 24 years (1981-2004) were analyzed for the effect of genetic group, season of calving, period of calving and parity of cow on cumulative part lactation milk yields. Heritabilities and genetic and phenotypic correlation coefficients were also estimated for these traits. The overall least square means estimated in this study for cumulative milk yields up to 30, 60,120,240 days were 267.46 ± 10.34kg, 548.82 ±21.57kg, 1061.27 ± 40.01kg and 1877.52 ± 59.98 kg respectively. The overall average for other traits viz. 305-days milk yield, total milk yield of lactation, lactation length, peak yield, calving interval, average milk yield per day of lactation length and average milk yield per day of calving interval were 2192.26±65.12kg, 2522.87±80.48kg, 375.11±4.02 days, 459.4±35.17days,12.76±0.36kg, 6.78±0.17kg and 5.61±0.16kg respectively. Cumulative part lactation milk yield traits were only affected by parity of cow. Genetic group, season of calving and period of calving were found to have no significant effect on these traits. The other traits viz. 305-days milk yield, total milk yield of lactation, lactation length, peak yield, calving interval, average milk yield per day of lactation length and average milk yield per day of calving interval, were not affected by genetic group, season of calving and period of calving and parity of cow except 305 days milk yield which was significantly affected by parity of cow. High heritability (0.54±0.19 of 30 days yield to 0.70±0.23 of 120 days yield) and high genetic and phenotypic correlations were observed among different part lactation milk yield traits. The correlation coefficients were moderate to high between part lactation milk yield traits and complete lactation milk yield traits. The high heritability of 60 days milk yield (0.68±0.22), 120 days milk yield (0.70±0.23) and moderate heritability of peak yield (0.324±0.15) and correlations of these traits with other complete lactation milk yield traits indicates that these traits can be utilized as reliable tools for early selection of dairy cows. Low heritabilities for calving interval and lactation length indicates that the length of these traits is much influenced by management and husbandry practices, which can be improved by better management practices.ThesisItem Open Access GENETIC CHARACTERIZATION OF NATIVE CHICKEN OF HIMACHAL PRADESH USING MOLECULAR MARKERS(CSKHPKV, Palampur, 06-07-11) REEN KOUR, JAGISH; Katoch, SanjeetABSTRACT Sixty five (65) blood samples at random from birds procured from different locations of the state and maintained at CSKHPKV Poultry farm, Palampur were collected. Fifteen microsatellite markers (MCW073, MCW005, MCW004, MCW051, MCW049, MCW048, MCW001, MCW014, ADL-158, ADL-172, ADL-267, ADL-210, ADL-171, ADL-176, ADL136) which gave amplification were included in the analysis. The PCR products for different microsatellite loci were resolved on 6% denaturing (urea) polyacrylamide gels along with 50 and 100 bp DNA ladders at 40-45V. Microsatellite alleles were visualized by silver staining. The observed alleles, effective number of alleles, gene frequency, observed heterozygosity, expected heterozygosity were estimated through POPGENE software. The mean observed and effective numbers of alleles were found to be 5.87 ± 0.28. and 4.19 ± 0.17 respectively across all loci. Total 88 alleles were observed with maximum alleles (8) contributed by locus MCW073, ADL-176 & and the least alleles (4) by (ADL-171, ADL-136, MCW014). The average heterozygosity was observed to be 0.76 ± 0.01 across all loci. The average observed and expected heterozygosity 0.56 ± .03 and 0.77 ± 0.01 respectively. The observed heterozygosity (0.8900) was highest for ADL-176, and least (0.4082) for ADL-267. The Expected heterozygosity (0.8278) was highest for MCW073 and least (0.6890) least for MCW014. All the microsatellites had shown high levels of genetic variability.The screened loci were not found to be in accordance with Hardy-Weinberg equilibrium for several microsatellite frequencies. Genes ChB6 and IL-2Rγ were used to study nucleotide polymorphism related to immunocompetence The PVU-II restriction enzyme digestion of 215bp amplicon of ChB6 gene generated fragments of 215,147 & 68 bp and revealed highly polymorphic patterns. The Hph1 restriction enzyme digestion of 600bp amplicon of IL-2Rγ gene generated fragments of 465,454,134,104,42bp and revealed highly polymorphic patterns. Fragment pattern revealed all the genes have high level of polymorphism in DNA samples of Native Chicken of Himachal Pradesh.