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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Animal Genetics and Breeding × End date: 2019 × Start date: 2015 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    MOLECULAR GENETIC STUDIES OF SOME GENES ASSOCIATED WITH FECUNDITY, GROWTH AND DISEASE RESISTANCE IN NATIVE SHEEP AND GOAT BREEDS OF HIMACHAL PRADESH
    (CSKHPKV, Palampur, 2018-07) Sankhyan, Varun; Thakur, Y.P.
    Sheep and goat husbandry is a predominant livestock activity in harsh climatic regions of the country. The present study was undertaken with the objectives to study genetic polymorphism of some genes related with fecundity, growth and disease resistance in native sheep and goat breeds of Himachal Pradesh. Genomic DNA was isolated from 63, 68, 197 and 73 animals belonging to Gaddi sheep, Rampur Bushair sheep, Gaddi goats and Chegu goats, respectively. Screening of different breeds for established ovine fecundity genes revealed that out of 8 point mutations screened for GDF9, BMP5, and BMPR1B genes, 2 point mutations, (G4-GDF9) and (G7-GDF9) were detected in Gaddi sheep and Rampur-Bushair sheep. PCR-RFLP screening of KISS1 gene in Gaddi goats revealed, frequency of A and T alleles as 0.43 and 0.57 and T allele was associated with litter size in Gaddi goats. PCR-RFLP of PRLR gene in Gaddi goats, revealed SNP (T92C). The SSCP analysis for Growth Hormone (GH) gene revealed 12 different SSCP patterns. Different variants of GH gene fragments revealed significant association with body weights and body measurements. PCR-RFLP analysis of Insulin like Growth Factor-1 (IGF-1) and Myostatin gene revealed SNP (G264C) and SNP (A70G), respectively. The AB heterozygote genotype of myostatin gene demonstrated significantly higher body weight at 3 and 12 month and body girth at 9 month of age. DRB3 gene revealed 2 alleles and 3 restriction digestion profiles and 4 alleles and 7 restriction digestion profiles for PstI and HaeIII digestion respectively. PCR-RFLP analysis of DQB gene using HaeIII revealed two alleles A and B with frequency ranging from 0.45 to 0.62 and 0.38 to 0.55 in different breeds. The present study generated genotypic data for genes related with fecundity, growth and disease resistance for native sheep and goat breeds of Himachal Pradesh, for which the genotypic characterization information was limited. The generated information could be a valuable input for conservation and future genetic improvement of the breeds.