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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Animal Genetics and Breeding × Author: REEN KOUR, JAGISH × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    GENETIC CHARACTERIZATION OF NATIVE CHICKEN OF HIMACHAL PRADESH USING MOLECULAR MARKERS
    (CSKHPKV, Palampur, 06-07-11) REEN KOUR, JAGISH; Katoch, Sanjeet
    ABSTRACT Sixty five (65) blood samples at random from birds procured from different locations of the state and maintained at CSKHPKV Poultry farm, Palampur were collected. Fifteen microsatellite markers (MCW073, MCW005, MCW004, MCW051, MCW049, MCW048, MCW001, MCW014, ADL-158, ADL-172, ADL-267, ADL-210, ADL-171, ADL-176, ADL136) which gave amplification were included in the analysis. The PCR products for different microsatellite loci were resolved on 6% denaturing (urea) polyacrylamide gels along with 50 and 100 bp DNA ladders at 40-45V. Microsatellite alleles were visualized by silver staining. The observed alleles, effective number of alleles, gene frequency, observed heterozygosity, expected heterozygosity were estimated through POPGENE software. The mean observed and effective numbers of alleles were found to be 5.87 ± 0.28. and 4.19 ± 0.17 respectively across all loci. Total 88 alleles were observed with maximum alleles (8) contributed by locus MCW073, ADL-176 & and the least alleles (4) by (ADL-171, ADL-136, MCW014). The average heterozygosity was observed to be 0.76 ± 0.01 across all loci. The average observed and expected heterozygosity 0.56 ± .03 and 0.77 ± 0.01 respectively. The observed heterozygosity (0.8900) was highest for ADL-176, and least (0.4082) for ADL-267. The Expected heterozygosity (0.8278) was highest for MCW073 and least (0.6890) least for MCW014. All the microsatellites had shown high levels of genetic variability.The screened loci were not found to be in accordance with Hardy-Weinberg equilibrium for several microsatellite frequencies. Genes ChB6 and IL-2Rγ were used to study nucleotide polymorphism related to immunocompetence The PVU-II restriction enzyme digestion of 215bp amplicon of ChB6 gene generated fragments of 215,147 & 68 bp and revealed highly polymorphic patterns. The Hph1 restriction enzyme digestion of 600bp amplicon of IL-2Rγ gene generated fragments of 465,454,134,104,42bp and revealed highly polymorphic patterns. Fragment pattern revealed all the genes have high level of polymorphism in DNA samples of Native Chicken of Himachal Pradesh.