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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20211
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Subject: Agricultural Biotechnology × Author: Shree, Bharti × End date: 2022 × Start date: 2020 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Influence of cold stress on expression of invertase and calcium-dependent protein kinase genes in chickpea (Cicer arietinum L.)
    (Palampur, 2021-11-12) Shree, Bharti; Sharma, Kamal Dev
    Chickpea (Cicer arietinum L.) is an important food grain legume. Chickpea is sensitive to cold and suffers substantial yield losses due to cold stress. Invertases hydrolyse sucrose into glucose and fructose and play an important role in plant growth and development as well as plants’ responses to various stresses including cold. In addition to invertases, calciumdependent protein kinases (CDPKs) modify gene expression via transcription factors to achieve systematic plant growth/development and reaction to stresses. Information on invertases and calcium-dependent protein kinases as well as the role of these genes in stress tolerance/susceptibility in chickpea is unavailable. In this study, 19 invertase genes (11 cellwall invertase, one vacuolar invertase and seven alkaline/neutral invertase genes) and 31 CDPKs genes were identified in the chickpea genome. These genes were located on 7 chickpea chromosomes. A comprehensive analysis of invertase as well as CDPK genes and proteins were performed, including gene structure, mRNA structure, cis-acting elements in the promoter regions, phylogeny, evolutionary relationships, gene duplication events, protein structure, motifs, domains, physiochemical properties, sub-cellular localization and interactions of invertases as well as CDPKs with other proteins. Phylogenetic analysis revealed that chickpea invertases were comprised of five major lineages whereas CDPKs had four lineages. The members within the same sub-groups shared conserved domains. Expression analysis revealed that all the invertase genes were functional in chickpea however, these genes expressed differentially in contrasting chickpea genotype under cold stress and Ascochyta blight infection. Expression analysis revealed that cell wall invertases were associated in cold tolerance whereas majority of the CaCDPK genes were involved in low temperature responses by tolerant as well as sensitive genotypes of chickpea. Invertase genes associated with Ascochyta blight resistance in chickpea were also identified. The study laid the foundation for unravelling the complexity of chickpea responses to cold and Ascochyta rabiei infection and develop protocols for mitigation of cold stress in chickpea.