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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Agricultural Biotechnology × Author: Rai, Sourav × Start date: 2020 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Cryopreservation studies in Callus culture of Arnebia euchroma
    (palampur, 2022-05-09) Rai, Sourav; Bhushan, Shashi
    Arnebia euchroma commonly known as Ratanjot belongs to Boraginaceae family. Arnebia species is known for rich source of shikonin and their derivatives have antibacterial, antifungal, anti-HIV, anti-inflammatory and several other medicinal properties. The genus Arnebia has been over exploited as a result of these characteristics, and it is now classified as a endangered species. These resources must be protected in order to be used on a large scale in the future, and this valuable treasure must be preserved. Callus was induced from the leaves with (75%) induction when cultured on MS medium augmented with IBA (1.02 mg/l) and BAP (2.25 mg/l) and multiplied on same medium. Leaf derived callus were used for the cryopreservation Therefore, a protocol is optimized for cryopreservation of callus using vitrification, encapsulation vitrification and desiccation technique. Callus was precultured on sucrose enriched medium containing 3% (w/v) and 13.7% (w/v) sucrose. Among all these techniques of cryopreservation highest survival rate (65%) was found in desiccation when precultured on 13.7% sucrose followed by 90 minutes desiccation time. Callus used for the cryopreservation was not revived after cryogenic treatment and necrosed after eight weeks of culture in encapsulation vitrification method. Hence, this method of cryopreservation was not found suitable for preserving the callus tissues of A. euchroma. Non cryopreserved callus showed the highest total Deoxyshikonin content i.e. 369.28 (µg/gm) and 301.31 (µg/gm) shikonin content was observed and Cryopreserved callus showed the highest Deoxyshikonin content i.e. 319.23 (µg/gm) and total shikonin content of 296 (µg/gm). In UPLC chromatography only deoxyshikonin content i.e. 65.05 (µg/gm) was observed in non cryopreserved callus and 39.07(µg/gm) content was found in cryopreserved callus.