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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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20192
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Subject: Agricultural Biotechnology × Author: Gaikwad, Dinesh Subhash ×
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    ThesisItemOpen Access
    Studies on chickpea (Cicer arietinum L.) defence to blight pathogen Ascochyta rabiei
    (palampur, 2019-07-30) Gaikwad, Dinesh Subhash; Sharma, Kamal Dev
    Chickpea (Cicer arietinum L.), a self-pollinated diploid (2n=2x=16) with haploid genome size of ∼740 Mbps, is an important food legume of subtropical and tropical regions and is believed to be originated in the Mediterranean region. In cool and humid regions of the world, chickpea blight caused by Ascochyta rabiei causes huge yield losses. Some accession of C. arientinum and wild Cicer species possess resistance to the disease, however molecular mechanism governing host resistance are poorly understood. With the aim to understand regulation of expression of genes involved in defence to A. rabiei, the expression of six defence genes [Phenylalanine ammonia lyase (PAL), Isoflavone reductase (IFR) Flavanone 3- hydroxylase (F3H), Pathogenesis related protein - 2b (PR-2b), Basic leucine zipper 24 (bzip24) and SNAKIN-2 (Antimicrobial peptides)] was elucidated in resistant and susceptible genotype of chickpea after inoculation (2 h, 12 h, 24 h, 36 h, 72 h) with A. rabiei. Gene expression was studied using quantitative Real-Time PCR and the expression was normalized with two reference genes, Clathrin adaptor complexes (CAC) and ATP-binding cassette transporter (ABCT). The sequences of the mRNAs were retrieved from National Center for Biotechnology Information database followed by identification of coding sequences (CDS) and design of primers. The genes (PAL, IFR, F3H) of the phenylpropanoid pathway that govern phytoalexin production expressed within 2 h after landing of the spores on chickpea surface and expression was considerably higher in resistant HC-1 then susceptible GPF2. The genes for pathogenesis related protein (PR-2b), antimicrobial peptide (SNAKIN-2) and transcription factor (bZIP24) had peaks at 24 h in the resistant genotype indicating maximum expression in resistant genotype at the time of host penetration and subsequent pathogen spread. The susceptible host (GPF2), on the other hand was slow in its response as is evident from low or delayed overexpression as compared to resistant host. It appears that defence to Ascochyta blight in chickpea is a precisely coordinated reaction of the host, where phytoalexin accumulation appears to occur within hours of inoculation, whereas antimicrobial peptides accumulate at the time of host invasion and pathogen spread
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    ThesisItemOpen Access
    STUDIES ON CHICKPEA (Cicer arietinum L.) DEFENCE TO BLIGHT PATHOGEN ASCOCHYTA RABIEI.
    (CSKHPKV, Palampur, 2019-07) Gaikwad, Dinesh Subhash; Sharma, Kamal Dev
    Chickpea (Cicer arietinum L.), a self-pollinated diploid (2n=2x=16) with haploid genome size of ∼740 Mbps, is an important food legume of subtropical and tropical regions and is believed to be originated in the Mediterranean region. In cool and humid regions of the world, chickpea blight caused by Ascochyta rabiei causes huge yield losses. Some accession of C. arientinum and wild Cicer species possess resistance to the disease, however molecular mechanism governing host resistance are poorly understood. With the aim to understand regulation of expression of genes involved in defence to A. rabiei, the expression of six defence genes [Phenylalanine ammonia lyase (PAL), Isoflavone reductase (IFR) Flavanone 3- hydroxylase (F3H), Pathogenesis related protein - 2b (PR-2b), Basic leucine zipper 24 (bzip24) and SNAKIN-2 (Antimicrobial peptides)] was elucidated in resistant and susceptible genotype of chickpea after inoculation (2 h, 12 h, 24 h, 36 h, 72 h) with A. rabiei. Gene expression was studied using quantitative Real-Time PCR and the expression was normalized with two reference genes, Clathrin adaptor complexes (CAC) and ATP-binding cassette transporter (ABCT). The sequences of the mRNAs were retrieved from National Center for Biotechnology Information database followed by identification of coding sequences (CDS) and design of primers. The genes (PAL, IFR, F3H) of the phenylpropanoid pathway that govern phytoalexin production expressed within 2 h after landing of the spores on chickpea surface and expression was considerably higher in resistant HC-1 then susceptible GPF2. The genes for pathogenesis related protein (PR-2b), antimicrobial peptide (SNAKIN-2) and transcription factor (bZIP24) had peaks at 24 h in the resistant genotype indicating maximum expression in resistant genotype at the time of host penetration and subsequent pathogen spread. The susceptible host (GPF2), on the other hand was slow in its response as is evident from low or delayed overexpression as compared to resistant host. It appears that defence to Ascochyta blight in chickpea is a precisely coordinated reaction of the host, where phytoalexin accumulation appears to occur within hours of inoculation, whereas antimicrobial peptides accumulate at the time of host invasion and pathogen spread.