Thumbnail Image

Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

Browse

20201
Reset filters
Subject: Agricultural Biochemistry × Author: SINGH, SHIPRA × Type: Thesis × Thesis Type: Ph.D ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    PHYTOCHEMICAL STUDIES OF VIRUS INFECTED CAPSICUM AND MOLECULAR CHARACTERISATION OF VIRAL PATHOGEN
    (CSKHPKV. Palampur, 2020-01-31) SINGH, SHIPRA; Sharma, Neelam
    Capsicum is a cash crop of Himachal Pradesh commonly known for the abundance of antioxidants. Viral disease incidence has become very prominent in this crop. A total 19 viruses are known to infect Capsicum crop in India. In the present investigation entitled, “Phytochemical studies of virus infected Capsicum and molecular characterisation of viral pathogen”, a survey was conducted and 51 leaf samples were collected on the basis of symptoms from three districts of Himachal Pradesh i.e. Kangra, Kullu and Mandi. All the collected samples were analysed for the presence of viruses and their effect on phytochemical constituents. In addition to this, molecular characterisation of most prevalent virus was carried out. Screening of the samples for the presence of viruses through DAS-ELISA and identification and confirmation through RT-PCR revealed that CMV, PVY, PVX, GBNV, PSTVd (viroid) and TYLCV were the common viruses which infect Capsicum crop in Himachal Pradesh. The percent viral infection in Capsicum was 58.8 percent for CMV, 37.2 percent for PVY, 7.8 percent for TYLCV, 29.4 percent for PVX and 1.96 percent for PSTVd and GBNV both. Thus, CMV was the most prevalent virus in Capsicum crop. A significant decrease was observed in virus infected leaf samples of Capsicum for antioxidant activity, total carotenoids, total chlorophyll and ascorbic acid content Whereas total phenols and total flavonoids increased in infected samples.The molecular characterisation of CMV was carried out. An amplicon of approximately 829 bp, 958 bp, 1,314 bp, 373 bp, 842 bp and 647 bp for 1a helicase,1a methyltransferase, 2a, 2b, 3a and 3b genes were obtained. BLASTn analysis revealed that 1a helicase protein gene showed 98.35 percent identity with 1a helicase isolate of Capsicum sp. from Germany,1a methyltransferase protein gene showed 98.21 percent identity with 1a methyltransferase protein gene isolate of Tagetus erecta. from India, 2a protein gene showed 99.39 percent identity with Cucumber mosaic virus isolate TC segment of RNA -2 from UK, 2b protein gene showed 94.07 percent identity with 2b protein genes from Germany, movement protein (3a) gene showed 99.28 percent identity with Cucumber mosaic virus isolate CMV12 3a protein (3a) gene of Zucchini from Poland and Coat protein (3b) gene showed 99.23 percent identity with CP gene for coat protein of Valeriana from India.Comparative genome sequence analysis and phylogenetic analysis of CMV genes based on subgroups revealed that CMV-PLP 8 isolate belongs to subgroup-II of CMV.