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Chaudhary Charan Singh Haryana Agricultural University, Hisar
Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.
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ThesisItem Open Access Survey, isolation and identification of bacterial diseases of fresh water fishes in district Hisar, Haryana(CCSHAU, 2005) Dahiya, Tejpal; Sihag, R.C.;The present investigation was carried out to identify fish pathogens causing fish diseases in catla, rohu, mrigal, common carp, puthi and magur. A number of biochemical tests were carried out for identification of bacterial fish pathogens. The bacterial diseases were identified viz. epizootic ulcerative syndrome, fin and tail rot, hemorrhagic septicemia and vibriosis in three selected fish farms (Mirka, Satrod and SLR, Hansi) from July 2004 to March 2005. These diseases were reported in July, August, October and November. No disease was found in September, December, January, Febuary and March. Ten gram negative ( Aeromonas hydrophila, Enterobacter cloacae, E. gergoviae, Pseudomonas sp., P. pseudomallie, Klebsiella pneumoniae subsp. aerogenes, Providencia sp., Serratia marcescens, Vibrio anguillarum, V. alginolyticus) and two gram positive (Micrococcus roseus, Streptococcus gp Q1) bacterial pathogens were identified from infected fishes. In EUS, fungal hyphae were found penetrating deep into muscles in catla. In fish farm of Satrod, vibriosis was reported in magur. The bacterial pathogens (Vibrio anguillarum, V. alginolyticus) were isolated from the skin lesions. These bacteria are generally found in marine, brackish and rarely in fresh water. Deviation of optimal range of hydrobiological parameters led to infection in fishes resulting in to disease outbreaks.ThesisItem Open Access Plant regeneration studies in guar patta (Aloe vera L.)(CCSHAU, 2005) Saroha, Vandanna; Yadav, Neelam R.The present study was conducted to develop micropropagation system in Aloe vera L. and establishment of the regenerated plantlets in the soil. Shoot tip and leaf explants from 4-5 cm long offshoots were collected from field grown plants. These explants were cultured on 18 different MS modified media fortified with different concentrations and combinations of auxin, cytokinins and additives. MS medium supplemented with TDZ (1.0 mg/l) produced highest shoot formation response and further upscaling was done on MS medium with BA (2.0 mg/l) and IAA (0.2 mg/l). For shoot elongation, Tyrosine (50.0 mg/l) alongwith BA (5.0 mg/l) and adenine sulphate (80 mg/l) in MS medium produced 158.5 per cent increase in shoot length along with rooting. Leaf explants did not respond to shoot formation, however, 24.9 per cent callusing was observed on medium with 2,4-D (1.0 mg/l). The regenerated shoots showed 100 per cent rooting in all the six media tried for rooting, but longest roots (9.16 cm) and highest number of roots per shoot (13.22) were observed on medium fortified with paclobutrazol (0.5 mg/l) after 45 days of transfer. The regenerated plants were kept for hardening in water overnight and transferred to the pots with soil and vermiculite mixture (1:1). The rooting medium also affected the survival rate of the regenerated plants in soil. Highest survival rate was observed in paclobutrazol rooted plantlets. The regenerated plants were analysed for homogeneity through SDS-PAGE and all the plants showed similar soluble protein banding pattern. Promising variant has been recorded among plants regenerated on paclobutrazol (0.5 mg/l) containing medium. It also contained around four times more soluble protein and one and a half times more sapogenin content than the field grown plants. It showed thin cuticle, succulent and broad leaves. These plants are under observation for their utility in gel extraction.ThesisItem Open Access Assessment of ground water quality of Kaithal and Guhla blocks of Kaithal district, Haryana(CCSHAU, 2016) Vijay Kant Singh; Ram PrakashKaithal, the north eastern district of Haryana State with a total geographical area of 2317 sq. km is located between 29 o 31’: 30 o 12’ North latitudes and 76 o 10’: 76 o 42’ East longitudes. To ascertain the quality of groundwater and its impact on chemical properties of soil, water samples were collected from all the villages of Kaithal and Guhla blocks and analyzed by standard methods. 241 water samples were collected and analyzed for pH, electrical conductivity (EC), soluble cations (Ca 2+ , Mg 2+ , Na + and K + ) and anions (CO 3 2− , HCO 3 − , Cl − , SO 4 2− and NO3 − ) by using standard procedures. The values of SAR and RSC of water samples were computed. The pH, EC, SAR and RSC in groundwater ranged from 7.36-9.80, 0.30-2.31 (dSm -1 ), 2.55-14.39 (mmol l -1 ) 1/2 and nil-5.80 (me l -1 ), respectively in Guhla block and from 7.25-9.25, 0.47-6.06 (dSm -1 ), 3.69-28.59 (mmol l -1 ) 1/2 , nil-5.70 (me l -1 ) in Kaithal block. According to AICRP classification, 63.2, 2.7, 11.1, 22.1 and 0.9% samples were found to be of good, marginally saline, marginally alkali, alkali and highly alkali, respectively in Guhla block and 31.1, 26.5, 8.3, 8.3, 9.9 and 15.9% were of good, marginally saline, high SAR saline, marginally alkali, alkali and highly alkali, respectively in Kaithal block. After categorization of water samples, 84 soil samples from 21 representative profiles were collected from depth, 0-15, 15-30, 30-45 and 45-60 cm which were analyzed for pH, EC e , CEC, ESP, available N, P, K, OC, CaCO 3, soluble cations (Ca 2+ , Mg 2+ , Na + , K + ) and anions (CO 3 2− , HCO 3 -, Cl − , SO 4 2− ,) by standard procedures. In Guhla block, lowest pH of saturation extract was found in village Sultania and highest in Kasauli, while in case of Kaithal block lowest in Titram village and highest in Mundri. In Guhla block, lowest EC e (1.20 dSm -1 ), SAR e 2.87 (mmol l -1 ) 1/2 and ESP (3.83) were found in Kharkhada village while highest ECe (4.63 dSm -1 ) in Bhagal, SAR e 16.65 (mmol l -1 ) 1/2 and ESP (20.31) in Dusharpur. In case of Kaithal block, lowest EC e (1.70 dSm -1 ) in Jagdishpura, SAR e 3.28 (mmol l -1 ) 1/2 and ESP (4.23) were in Titram while highest EC e 6.67 (dSm -1 ) in Khurana, SAR e 20.33 (mmol l -1 ) 1/2 and ESP (25.55) in Ujana village. In Guhla block, lowest CEC 6.66-10.07 (cmol(+)kg -1 ) observed in Kasauli village and highest 13.82-15.26 (cmol(+)kg -1 ) in Bhagal village. In Kaithal block, lowest 5.37-8.73 (cmol(+)kg -1 ) in Mundri and highest 11.57-14.24 (cmol(+)kg -1 ) in Chandana village. Soil profiles were collected based on different categories of irrigation water. Most of the profiles were having salt developed due to the presence of salts in irrigation water. Most of the soil profiles were low in fertility. Highest electrical conductivity and sodium adsorption ratio of saturation extract were observed in surface layers of the soil which gradually decreased with depth irrespective of quality of irrigation water. After the analysis of soil and water, the Arc Map 9.3 was used to create various thematic maps like pH, EC, SAR and RSC of the study area. These thematic maps were combined to prepare water quality maps.